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产品描述：人血管内皮细胞生长因子B(VEGF-B)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的VEGFB含量。VEGFB是血管内皮生长因子家族成员。其背景上，在血管生成等生理过程中有一定关联。研究机制方面，它可通过与受体结合，调控内皮细胞的活动，在血管发育、维持血管稳态等方面发挥作用，目前也是血管相关疾病研究的潜在靶点。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，广泛应用于心血管疾病机制研究、代谢相关疾病模型评估、神经退行性病变干预实验等科研场景，尤其适用于探究VEGF - B在组织修复、能量代谢调控及疾病微环境中的作用机制，为体外实验提供可靠的蛋白定量解决方案。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Vascular endothelial growth factor B ELISA Kit; Vascular endothelial growth factor-related factor ELISA Kit; VEGF B ELISA Kit; VEGF related factor ELISA Kit; VEGF-B ELISA Kit; VEGF-related factor ELISA Kit; Vegfb ELISA Kit; VEGFB_HUMAN ELISA Kit; VEGFL ELISA Kit; VRF ELISA Kit
缩写：VEGFB
Uniprot No.：P49765
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human VEGF-B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 88 | ||||
| Range % | 80-96 | |||||
| 1:2 | Average % | 100 | ||||
| Range % | 95-104 | |||||
| 1:4 | Average % | 99 | ||||
| Range % | 92-110 | |||||
| 1:8 | Average % | 89 | ||||
| Range % | 86-96 | |||||
回收率：
| The recovery of human VEGF-B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 99 | 95-105 | ||||
| EDTA plasma (n=4) | 96 | 92-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected10002.6972.5122.6052.4635002.3222.2012.2622.1202501.5461.4981.5221.3801250.8150.7890.8020.66062.50.4170.4100.4140.27231.20.2810.2760.2790.13715.60.2190.2130.2160.07400.1430.1400.142 | ||||||
货期：3-5 working days
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