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Human tumor necrosis factor-related apoptosis-inducing ligand receptor 4(TRAIL-R4) ELISA Kit

  • 中文名： 人肿瘤坏死因子相关凋亡诱导配体受体4(TRAIL-R4)ELISA Kit
货号: IPD-E04749h
Price： ￥2500
数量：
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产品详情

  • 产品描述：
    人肿瘤坏死因子相关凋亡诱导配体受体4(TRAIL-R4)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的TNFRSF10D含量。TNFRSF10D是一个靶点。它在肿瘤免疫等领域有重要背景，可参与调节免疫细胞活性等过程。其研究机制方面，主要围绕它与配体相互作用、下游信号通路传导展开，影响细胞增殖、凋亡等，为相关疾病治疗研究提供了方向。试剂盒检测范围为0.156 ng/ml- 10 ng/ml，灵敏度为0.039 ng/ml。该产品适用于肿瘤生物学、免疫调控机制等基础研究领域，可用于评估细胞模型、动物实验或临床前研究中TRAIL-R4的表达动态，辅助探究其在肿瘤微环境中的作用或药物干预效果，例如化疗药物敏感性测试、凋亡信号通路研究等场景。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    CD 264 ELISA Kit; CD264 ELISA Kit; DcR 2 ELISA Kit; DcR2 ELISA Kit; Decoy receptor 2 ELISA Kit; Decoy with truncated death domain ELISA Kit; OTTHUMP00000123528 ELISA Kit; TNF receptor related receptor for TRAIL ELISA Kit; TNF related apoptosis inducing ligand receptor 4 ELISA Kit; TNF-related apoptosis-inducing ligand receptor 4 ELISA Kit; TNFRSF 10D ELISA Kit; TNFRSF10D ELISA Kit; TR10D_HUMAN ELISA Kit; TRAIL R4 ELISA Kit; TRAIL receptor 4 ELISA Kit; TRAIL receptor with a truncated death domain ELISA Kit; TRAIL-R4 ELISA Kit; TRAILR4 ELISA Kit; TRUNDD ELISA Kit; Tumor necrosis factor receptor superfamily member 10D ELISA Kit; Tumor necrosis factor receptor superfamily; member 10d; decoy with truncated death domain ELISA Kit
  • 缩写：
    TNFRSF10D
  • Uniprot No.：
    Q9UBN6
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.156 ng/ml - 10 ng/ml
  • 灵敏度：
    0.039 ng/ml
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cell Biology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human TRAIL-R4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    89


    Range %

    86-92


    1:2

    Average %

    95


    Range %

    91-99


    1:4

    Average %

    98


    Range %

    92-104


    1:8

    Average %

    92


    Range %

    82-95


  • 回收率：

    The recovery of human TRAIL-R4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    95

    90-101


    EDTA plasma (n=4)

    107

    102-110


  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    ng/ml

    OD1

    OD2

    Average

    Corrected


    10

    2.214

    2.323

    2.269

    2.112


    5

    1.597

    1.486

    1.542

    1.385


    2.5

    0.957

    0.886

    0.922

    0.765


    1.25

    0.547

    0.568

    0.558

    0.401


    0.625

    0.355

    0.366

    0.361

    0.204


    0.312

    0.281

    0.297

    0.289

    0.132


    0.156

    0.208

    0.214

    0.211

    0.054


    0

    0.156

    0.157

    0.157




  • 货期：
    3-5 working days

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