关键信息
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基因名
PRKAR1A
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简介
PRKAR1A protein is a regulatory subunit of cAMP-dependent protein kinase and is critical for cAMP signaling. Upon activation by cAMP, it releases two catalytic monomers and a regulatory dimer from the inactive holoenzyme. PRKAR1A Protein, Human (HEK293, His) is the recombinant human-derived PRKAR1A protein, expressed by HEK293 , with C-6*His labeled tag.
- 应用
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别名
Tissue-specific extinguisher 1; TSE1
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种属
Human
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表达系统
HEK293
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标签
C-6*His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P10644
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表达区间
E2-V381
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氨基酸序列
ESGSTAASEEARSLRECELYVQKHNIQALLKDSIVQLCTARPERPMAFLREYFERLEKEEAKQIQNLQKAGTRTDSREDEISPPPPNPVVKGRRRRGAISAEVYTEEDAASYVRKVIPKDYKTMAALAKAIEKNVLFSHLDDNERSDIFDAMFSVSFIAGETVIQQGDEGDNFYVIDQGETDVYVNNEWATSVGEGGSFGELALIYGTPRAATVKAKTNVKLWGIDRDSYRRILMGSTLRKRKMYEEFLSKVSILESLDKWERLTVADALEPVQFEDGQKIVVQGEPGDEFFIILEGSAAVLQRRSENEEFVEVGRLGPSDYFGEIALLMNRPRAATVVARGPLKCVKLDRPRFERVLGPCSDILKRNIQQYNSFVSLSV
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蛋白长度
Partial
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分子量
45-55 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
PRKAR1A, or Protein Kinase cAMP-Dependent Protein Kinase Regulatory Subunit Type I Alpha, is an essential component of the protein kinase A (PKA) signaling pathway, which plays a critical role in various cellular processes such as metabolism, gene expression, and cell proliferation. Mutations or dysregulation of the PRKAR1A gene are linked to a spectrum of diseases, most notably Carney complex, characterized by multiple neoplasms and endocrine tumors. Research into PRKAR1A recombinant proteins has gained momentum in the context of understanding its structural and functional properties, as well as its interactions within the PKA signaling cascade. By producing and studying PRKAR1A as a recombinant protein, scientists aim to elucidate its regulatory mechanisms, how it interacts with catalytic subunits of PKA, and its role in disease pathology. This approach holds the potential for discovering novel therapeutic targets and strategies for conditions associated with PRKAR1A abnormalities, thereby offering insights into cellular signaling pathways and their implications in oncology and endocrinology. Through structural studies, including crystallography and NMR spectroscopy, researchers can provide a detailed understanding of the protein's conformation and interaction with ligands, contributing to the broader field of signal transduction and targeted therapies.
