首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠单核细胞趋化蛋白3(MCP-3/CCL7)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CCL7含量。CCL7，又称单核细胞趋化蛋白3（MCP3），是一种小细胞因子，主要在成纤维细胞、单核细胞和肿瘤细胞中表达。CCL7通过与其受体CCR1、CCR2、CCR3等结合，参与免疫细胞募集、钙离子内流和肿瘤转移等生物效应。其研究机制涉及CCL7与巨噬细胞极化的关系，以及其在类风湿关节炎等免疫疾病中的作用。试剂盒检测范围为1.56 pg/mL-100 pg/mL，适用于基础科研中炎症相关疾病模型（如感染、自身免疫性疾病或癌症）的机制研究，可通过定量分析MCP - 3/CCL7在生物样本中的动态变化，探索其与免疫细胞浸润、组织损伤修复或肿瘤进展的关联性，为药物干预效果评价及信号通路研究提供实验支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Ccl7 ELISA kit; Fic ELISA kit; Mcp3 ELISA kit; Scya7C-C motif chemokine 7 ELISA kit; Intercrine/chemokine MARC ELISA kit; Monocyte chemoattractant protein 3 ELISA kit; Monocyte chemotactic protein 3 ELISA kit; MCP-3 ELISA kit; Protein FIC ELISA kit; Small-inducible cytokine A7 ELISA kit
缩写：CCL7
Uniprot No.：Q03366
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：1.56 pg/mL-100 pg/mL
灵敏度：0.39 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse MCP-3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 95 | ||||
| Range % | 86-99 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 90-101 | |||||
| 1:4 | Average % | 93 | ||||
| Range % | 85-96 | |||||
| 1:8 | Average % | 97 | ||||
| Range % | 92-101 | |||||
回收率：
| The recovery of mouse MCP-3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 88 | 84-92 | ||||
| EDTA plasma (n=4) | 100 | 91-104 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 1002.6102.5002.5552.448 502.1972.1252.1612.054 251.4511.4661.4591.352 12.50.7500.7760.7630.656 6.250.3460.3400.3430.236 3.120.2400.2330.2370.130 1.560.1680.1590.1640.057 00.1060.1080.107 | ||||||
货期：3-5 working days
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