首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠血管内皮细胞生长因子B(VEGF-B)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的VEGFB含量。VEGFB即血管内皮生长因子B，是VEGF家族成员。其广泛表达于心脏、骨骼肌等组织。研究机制上，VEGFB主要通过与受体结合，调控血管内皮细胞等的生长、存活与迁移，在血管生成等生理病理过程中发挥重要作用，为相关疾病治疗提供潜在靶点。试剂盒检测范围为7.8 pg/mL-500 pg/mL，本试剂盒适用于体外培养细胞模型、动物组织样本及体液中的VEGF - B水平分析，可满足心血管疾病模型构建、血管生成机制研究及代谢相关基础实验的定量检测需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Vegfb ELISA kit; VrfVascular endothelial growth factor B ELISA kit; VEGF-B ELISA kit; VEGF-related factor ELISA kit; VRF ELISA kit
缩写：VEGFB
Uniprot No.：P49766
种属：Mus musculus (Mouse)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：7.8 pg/mL-500 pg/mL
灵敏度：0.195 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse VEGF-B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 97 | ||||
| Range % | 92-102 | |||||
| 1:2 | Average % | 91 | ||||
| Range % | 84-97 | |||||
| 1:4 | Average % | 90 | ||||
| Range % | 87-94 | |||||
| 1:8 | Average % | 100 | ||||
| Range % | 95-103 | |||||
回收率：
| The recovery of mouse VEGF-B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 87-93 | ||||
| EDTA plasma (n=4) | 93 | 90-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected5002.7432.7632.7532.5892502.4052.4352.4202.2561251.7561.7961.7761.61262.50.9781.0160.9970.83331.20.5010.5350.5180.35415.60.3510.3590.3550.1910.780.2370.2550.2460.08200.1620.1650.164 | ||||||
货期：3-5 working days
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