首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠基质细胞衍生因子1α(SDF-1α/SDF1A)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的SDF1A/CXCL12含量。SDF1A/CXCL12是重要靶点。它属于趋化因子，在胚胎发育、免疫调节等生理过程中起关键作用。研究机制上，其主要通过与受体CXCR4等结合激活下游信号通路，影响细胞迁移、增殖等，在肿瘤转移、炎症等病理进程研究多。试剂盒检测范围为0.156 ng/mL-10 ng/mL，广泛应用于免疫调控研究、肿瘤微环境分析、心血管疾病模型构建等科研领域，为体外研究SDF - 1α介导的细胞迁移、血管生成等生物学过程提供可靠的检测工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Cxcl12 ELISA Kit; Sdf1 ELISA Kit; Stromal cell-derived factor 1 ELISA Kit; SDF-1 ELISA Kit; 12-O-tetradecanoylphorbol 13-acetate repressed protein 1 ELISA Kit; TPAR1 ELISA Kit; C-X-C motif chemokine 12 ELISA Kit; Pre-B cell growth-stimulating factor ELISA Kit; PBSF ELISA Kit; Thymic lymphoma cell-stimulating factor ELISA Kit; TLSF ELISA Kit
缩写：SDF-1α/SDF1A
Uniprot No.：P40224
种属：Mus musculus (Mouse)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse SDF-1α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 101 | ||||
| Range % | 99-105 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 91-98 | |||||
| 1:4 | Average % | 89 | ||||
| Range % | 84-94 | |||||
| 1:8 | Average % | 98 | ||||
| Range % | 95-104 | |||||
回收率：
| The recovery of mouse SDF-1α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 98 | 95-101 | ||||
| EDTA plasma (n=4) | 92 | 87-97 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected102.3582.4672.4132.25852.0021.9771.9901.8352.51.3551.4251.3901.2351.250.8150.8580.8370.6820.6250.5100.5250.5180.3630.3120.3450.3730.3590.2040.1560.2710.2800.2760.12100.1560.1530.155 | ||||||
货期：3-5 working days
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