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产品描述：仓鼠假定磷脂酶B样蛋白2(PLBD2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的PLBD2含量。仓鼠PLBD2是一种假定的磷脂酶，与IGF2R相互作用，可能参与生长和信号传导。其磷脂酶活性尚不明确，但研究可能揭示其在细胞稳态和调节机制中的作用。试剂盒检测范围为12.5 pg/mL-800 pg/mL，科研人员可将其应用于基础研究领域，例如探索PLBD2在脂质代谢途径中的功能机制、评估特定疾病模型中该蛋白的表达动态变化，或用于细胞信号转导相关实验的分子标记物检测。适用于分子生物学实验室常规蛋白定量研究需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：P76, LAMA-like protein 2|PLB homolog 2|lamina ancestor homolog 2|mannose-6-phosphate protein associated protein p76|phospholipase B-like 2 32 kDa form|phospholipase B-like 2 45 kDa form
缩写：PLBD2
种属：Mesocricetus auratus (Golden hamster)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：12.5 pg/mL-800 pg/mL
灵敏度：3.12 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of hamster PLBD2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 94 | ||||
| Range % | 90-105 | |||||
| 1:2 | Average % | 92 | ||||
| Range % | 86-96 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 90-100 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 91-100 | |||||
回收率：
| The recovery of hamster PLBD2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 87-94 | ||||
| EDTA plasma (n=4) | 96 | 89-103 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected8002.3492.3672.3582.2274001.9751.9241.9501.8192001.4081.4661.4371.3061000.9120.8570.8850.754500.5550.5210.5380.407250.3630.3740.3690.23812.50.2480.2590.2540.12300.1330.1290.131 | ||||||
货期：3-5 working days
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