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产品描述：鱼细胞色素P450 1A1(CYP1A1)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CYP1A1含量。CYP1A1是一种主要的细胞色素P450酶，广泛研究其激活致癌化合物的能力。它通过芳香烃受体（AhR）介导的转录激活，在代谢环境致癌物和膳食化合物中发挥重要作用。CYP1A1在肺癌等癌症的发生发展中扮演关键角色，其研究机制涉及致癌物激活、基因多态性、以及与AhR等受体的相互作用。试剂盒检测范围为75 pg/mL-1200 pg/mL，适用于科研场景中评估鱼类暴露于环境毒素后的生理响应、污染物生物有效性研究以及生态毒理学模型的建立。为水生生态系统健康监测、污染物代谢机制研究及水产养殖安全评估提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：cyp1a1 ELISA kit; cyp1aCytochrome P450 1A1 ELISA kit; EC 1.14.14.1 ELISA kit; CYPIA1 ELISA kit
缩写：CYP1A1
Uniprot No.：Q6JZS3
种属：Fish
样本类型：serum, plasma, tissue homogenates
检测范围：75 pg/mL-1200 pg/mL
灵敏度：75 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of fish CYP1A1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 91 | ||||
| Range % | 86-98 | |||||
| 1:2 | Average % | 100 | ||||
| Range % | 92-107 | |||||
| 1:4 | Average % | 85 | ||||
| Range % | 80-91 | |||||
| 1:8 | Average % | 88 | ||||
| Range % | 83-96 | |||||
回收率：
| The recovery of fish CYP1A1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 89 | 88-95 | ||||
| EDTA plasma (n=4) | 94 | 91-97 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2Average 12000.1460.1470.147 6000.2380.2540.246 3000.5240.5530.539 1501.1121.1351.124 752.3382.2772.308 | ||||||
货期：3-5 working days
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