首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠降钙素基因相关肽(CGRP)酶联免疫试剂盒检测血清、血浆、组织匀浆样本中的CGRP含量。CGRP（降钙素基因相关肽）是一种神经肽，主要在感觉神经纤维表达，如C和Aδ感觉纤维，广泛分布于全身，尤其在血管周围。CGRP在偏头痛的发病机制中起关键作用，通过促进神经源性炎症和颅脑血管扩张引发偏头痛。近年来，针对CGRP及其受体的药物研发成为偏头痛治疗的热点。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，支持科研人员探究CGRP在神经科学、药理学或疾病模型中的动态变化，例如分析疼痛模型中神经递质释放规律、评估药物对CGRP通路的调控作用，或研究心血管疾病与神经肽关联机制。本试剂盒适用于多种生物样本类型，为神经生物学、免疫学及代谢性疾病等基础研究领域提供标准化检测工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Beta type CGRP、Calcitonin gene-related peptide II、CGRPII、Calcitonin gene-related peptide 2、CALCB
缩写：CGRP
Uniprot No.：Q99MP3
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse CGRP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||
| Sample | Serum(n=4) | ||
| 1:1 | Average % | 94 | |
| Range % | 86-98 | ||
| 1:2 | Average % | 92 | |
| Range % | 88-97 | ||
| 1:4 | Average % | 90 | |
| Range % | 85-96 | ||
| 1:8 | Average % | 94 | |
| Range % | 88-99 |
回收率：
| The recovery of mouse CGRP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 89-97 | ||||
| EDTA plasma (n=4) | 96 | 91-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 10002.3422.3212.3322.178 5001.7721.7601.7661.612 2501.2501.2411.2461.092 1250.7990.7890.7940.640 62.50.4990.4870.4930.339 31.250.3640.3410.3530.199 15.60.2530.2440.2490.095 00.1560.1520.154 | ||||||
货期：3-5 working days
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