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产品描述：人6-羟基硫酸褪黑素(6HMS)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的6HMS含量。人6-羟基硫酸褪黑素（6HMS）是褪黑素的代谢产物，参与调节生物节律和抗氧化作用。研究发现，6HMS在妊娠期高血压疾病（HDCP）中发挥重要作用，通过调节胎盘氧化应激水平和内皮功能，参与HDCP的发病机制。试剂盒检测范围为10 pg/mL- 400 pg/mL，可应用于褪黑素代谢通路研究、神经内分泌系统功能分析，或探索6HMS在睡眠障碍、神经退行性疾病及免疫调节中的潜在作用机制，为体外实验提供稳定可靠的检测工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：6HMS
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：10 pg/mL- 400 pg/mL
灵敏度：10 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human 6HMS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 95 | ||||
| Range % | 92-98 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 87-105 | |||||
| 1:4 | Average % | 96 | ||||
| Range % | 89-102 | |||||
| 1:8 | Average % | 92 | ||||
| Range % | 88-96 | |||||
回收率：
| The recovery of human 6HMS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 89-100 | ||||
| EDTA plasma (n=4) | 95 | 91-101 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2Average 4000.1540.1560.155 2000.3600.3420.351 920.5720.5430.558 300.8560.8600.858 101.4751.4891.482 02.3412.4352.388 | ||||||
货期：3-5 working days
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