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产品描述：犬补体蛋白3(C3)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的C3含量。C3是一种重要靶点。它在补体系统中扮演关键角色，参与免疫防御等生理过程。研究发现其异常激活与多种疾病相关，如自身免疫病、肾脏疾病等。科研人员探究其激活机制，期望通过干预C3来开发新的治疗手段。试剂盒检测范围为0.78 μg/mL-50 μg/mL，本试剂盒适用于实验动物模型研究、免疫病理机制探索及药物干预效果评价等科研场景，例如通过检测感染性疾病或免疫相关疾病模型中C3的浓度变化，揭示补体系统在犬类疾病发生发展中的作用，为兽医学研究提供可靠的数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：AHUS5, ARMD9, ASP, CPAMD1, acylation-stimulating protein cleavage product|complement component C3
缩写：C3
种属：Canis lupus familiaris (Dog) (Canis familiaris)
样本类型：serum, plasma, tissue homogenates
检测范围：0.78 μg/mL-50 μg/mL
灵敏度：0.195 μg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of dog C3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 99 | ||||
| Range % | 95-102 | |||||
| 1:200 | Average % | 95 | ||||
| Range % | 81-89 | |||||
| 1:400 | Average % | 92 | ||||
| Range % | 88-96 | |||||
| 1:800 | Average % | 101 | ||||
| Range % | 98-103 | |||||
回收率：
| The recovery of dog C3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 91-97 | ||||
| EDTA plasma (n=4) | 89 | 85-93 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| μg/mlOD1OD2Average500.0750.0790.077250.1300.1360.13312.50.2060.2040.2056.250.3220.3320.3273.120.5430.5380.5411.560.8510.8780.8650.781.1631.1981.18102.0142.0362.025 | ||||||
货期：3-5 working days
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