首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人溶血磷脂酸(LPA)酶联免疫试剂盒，竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的LPA含量。LPA（溶血磷脂酸）是一种生物活性磷脂介质，在体内参与多种生物学过程，如细胞增殖、分化、凋亡和迁移等。其研究机制涉及通过LPA受体激活多条信号通路，影响细胞功能。LPA在肿瘤、心血管疾病和骨组织工程等领域具有潜在应用价值。试剂盒检测范围为3.9 ng/mL-250 ng/mL，适用于基础研究中LPA代谢通路的动态监测、疾病模型中的生物标志物探索以及药物干预实验的效果评估。尤其适合细胞培养上清、动物模型组织等复杂基质中LPA的定量分析，为脂质介导的信号传导机制研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：LPA
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：3.9 ng/mL-250 ng/mL
灵敏度：3.9 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human LPA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 96 | ||||
| Range % | 92-102 | |||||
| 1:200 | Average % | 94 | ||||
| Range % | 91-98 | |||||
| 1:400 | Average % | 102 | ||||
| Range % | 98-106 | |||||
| 1:800 | Average % | 87 | ||||
| Range % | 82-95 | |||||
回收率：
| The recovery of human LPA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 88 | 80-96 | ||||
| EDTA plasma (n=4) | 96 | 92-102 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2Average 2500.0900.0890.090 1250.1470.1540.151 62.50.2550.2670.261 31.250.5000.4910.496 15.60.7570.7620.760 7.81.0681.0591.064 3.91.5441.4471.496 02.6232.6552.639 | ||||||
本试剂盒所含材料：
A 96-well Assay plate --The 96-well plate has been pre-coated with human LPA.
Standard(10 x concentrate) (1 x 200 μl) --Dilute the standard at dilution series, read the OD values, and then draw a standard curve.
HRP-conjugated LPA antibody(100 x concentrate) (1 x 60 μl) --Bind to the LPAA, and HRP catalyzes the TMB to elicit a chromogenic reaction.
HRP-conjugate Diluent (1 x 10 ml) --Dilute the HRP-conjugated LPAantibody solution.
Sample Diluent (2 x 20 ml) --Reconstitute the standard and dilute the sample to an appropriate concentration.
Wash Buffer (25x concentrate) (1 x 20 ml) --Wash away unbound or free substances.
本试剂盒不含材料：
A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm - 570 nm.
An incubator that can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
货期：3-5 working days
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