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产品描述:猪肝炎病毒细胞的受体1/肾损伤分子1/KIM1(HAVCR1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆样本中的HAVCR1含量。HAVCR1(甲型肝炎病毒细胞受体1)是一种跨膜糖蛋白,作为甲型肝炎病毒的受体,其研究揭示了HAV进入细胞的关键机制。HAVCR1的发现为甲型肝炎的治疗提供了新的靶点,通过研究其作用机制,有助于开发新型抗病毒药物。试剂盒检测范围为0.18 ng/mL-12 ng/mL,本产品适用于科研领域,如建立肝炎或肾损伤动物模型后评估靶蛋白动态变化、分析疾病相关信号通路调控机制,或用于筛选潜在治疗药物时监测生物标志物水平,为相关基础研究提供可靠数据支持。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
缩写:HAVCR1
种属:Sus scrofa (Pig)
样本类型:serum, plasma, tissue homogenates
检测范围:0.18 ng/mL-12 ng/mL
灵敏度:0.04 ng/mL
反应时间:1-5h
样本体积:50-100ul
检测波长:450 nm
研究领域:Microbiology
测定原理:quantitative
测定方法:Sandwich
精密度:
Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
Inter-assay Precision (Precision between assays): CV%<10% | |||||||
Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of pig HAVCR1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
Sample | Serum(n=4) | ||||||
1:1 | Average % | 92 | |||||
Range % | 85-101 | ||||||
1:2 | Average % | 93 | |||||
Range % | 82-102 | ||||||
1:4 | Average % | 95 | |||||
Range % | 88-99 | ||||||
1:8 | Average % | 95 | |||||
Range % | 90-105 |
回收率:
The recovery of pig HAVCR1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
Sample Type | Average % Recovery | Range | |||||
Serum (n=5) | 95 | 86-100 | |||||
EDTA plasma (n=4) | 96 | 90-110 | |||||
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
ng/mlOD1OD2AverageCorrected121.7861.7931.7901.65361.4051.4561.4311.29431.0341.0081.0210.8841.50.6690.6820.6760.5390.750.4350.4480.4420.3050.3750.2880.3030.2960.1590.180.1850.1910.1880.05100.1350.1380.137 |
货期:3-5 working days
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