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产品描述：小鼠血清淀粉样蛋白A3(SAA3)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的SAA3含量。SAA3（血清淀粉样蛋白A-3）是一种重要的急性期反应蛋白，在人体应对急性生理挑战时发挥关键作用。它作为高密度脂蛋白（HDL）复合物中的载脂蛋白，参与脂质代谢调节，并在急性期反应和抗菌防御中具有重要作用。研究显示，SAA3在阿尔茨海默病模型小鼠中缺失可改善认知功能障碍和Tau蛋白病理进展。试剂盒检测范围为0.156 ng/mL-10 ng/mL，该产品可广泛应用于炎症机制研究、代谢性疾病动物模型评估、病原体感染后宿主反应监测等科研领域，为探索SAA3在先天免疫调控及病理过程中的功能提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Saa3 ELISA Kit; Serum amyloid A-3 protein ELISA Kit
缩写：SAA3
Uniprot No.：P04918
种属：Mus musculus (Mouse)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse SAA3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:100 | Average % | 97 | |||||
| Range % | 92-101 | ||||||
| 1:200 | Average % | 95 | |||||
| Range % | 90-100 | ||||||
| 1:400 | Average % | 91 | |||||
| Range % | 85-95 | ||||||
| 1:800 | Average % | 87 | |||||
| Range % | 82-91 | ||||||
回收率：
| The recovery of mouse SAA3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 103 | 99-112 | |||||
| EDTA plasma (n=4) | 84 | 80-88 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected102.8152.6582.7372.62751.9471.8931.9201.8102.51.2011.1451.1731.0631.250.6780.6810.6800.5700.6250.3840.3720.3780.2680.3120.2690.2630.2660.1560.1560.1730.1800.1770.06700.1120.1070.110 | |||||||
货期：3-5 working days
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