| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CD177 |
| Uniprot No | Q8N6Q3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 22-407aa |
| 氨基酸序列 | LLCQFGTVQHVWKVSDLPRQWTPKNTSCDSGLGCQDTLMLIESGPQVSLV LSKGCTEAKDQEPRVTEHRMGPGLSLISYTFVCRQEDFCNNLVNSLPLWA PQPPADPGSLRCPVCLSMEGCLEGTTEEICPKGTTHCYDGLLRLRGGGIF SNLRVQGCMPQPGCNLLNGTQEIGPVGMTENCNRKDFLTCHRGTTIMTHG NLAQEPTDWTTSNTEMCEVGQVCQETLLLLDVGLTSTLVGTKGCSTVGAQ NSQKTTIHSAPPGVLVASYTHFCSSDLCNSASSSSVLLNSLPPQAAPVPG DRQCPTCVQPLGTCSSGSPRMTCPRGTTHCYDGYIHLSGGGLSTKMSIQG CVAQPSSFLLNHTRQIGIFSAREKRDVQPPASQHEGVDHHHHHH |
| 预测分子量 | 47 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CD177重组蛋白的3-4篇参考文献示例(文献为假设性概括，供参考)：
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1. **文献名称**：*Recombinant expression and functional characterization of human CD177 in neutrophil extracellular trap formation*
**作者**：Smith A, et al.
**摘要**：该研究通过哺乳动物表达系统成功表达并纯化了重组CD177蛋白，证实其与PR3(蛋白酶3)的结合能力，并发现CD177过表达可增强中性粒细胞胞外诱捕网(NETs)的形成，提示其在炎症反应中的作用机制。
2. **文献名称**：*Structural insights into CD177-mediated recognition of PR3 in ANCA-associated vasculitis*
**作者**：Bauer S, et al.
**摘要**：利用X射线晶体学解析了重组CD177蛋白与PR3的复合物结构，揭示了CD177的特定结构域在自身抗体识别中的关键作用，为抗中性粒细胞胞浆抗体(ANCA)相关血管炎的靶向治疗提供依据。
3. **文献名称**：*CD177 recombinant protein as a diagnostic biomarker for myeloproliferative neoplasms*
**作者**：Li Y, et al.
**摘要**：通过ELISA和流式细胞术验证重组CD177蛋白在骨髓增生性肿瘤患者中的表达异常，证明其可作为潜在的诊断标志物，并探讨其与JAK2基因突变的相关性。
4. **文献名称**：*Optimization of CD177 recombinant production in E. coli for antigenic studies*
**作者**：Zhang R, et al.
**摘要**：研究通过大肠杆菌表达系统优化CD177重组蛋白的胞内可溶性表达和纯化工艺，证实其与ANCA阳性血清的免疫反应性，为开发相关检测试剂奠定基础。
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**注**：以上文献为示例性内容，实际引用时需以真实发表的论文为准。建议通过PubMed、Web of Science等平台以关键词“CD177 recombinant”或“CD177 protein expression”检索最新研究。
CD177 recombinant protein is a biologically engineered molecule designed to study the function and interactions of CD177. a cell surface glycoprotein primarily expressed on neutrophils. CD177. also known as HNA-2a or NB1 glycoprotein, is a member of the urokinase-type plasminogen activator receptor (uPAR) family and is anchored to the membrane via glycosylphosphatidylinositol (GPI). It plays a role in neutrophil migration, adhesion, and activation, particularly in inflammatory and autoimmune conditions. CD177 interacts with β2 integrins and is implicated in pathologies such as autoimmune vasculitis, where anti-CD177 autoantibodies are observed.
The recombinant CD177 protein is typically produced in mammalian or insect cell systems to ensure proper post-translational modifications, such as glycosylation, which are critical for its biological activity. It often includes a fusion tag (e.g., Fc or His-tag) for purification and detection. Researchers use this protein to investigate CD177-mediated signaling pathways, neutrophil-endothelial cell interactions, and its association with diseases like anti-neutrophil cytoplasmic antibody (ANCA)-associated vasculitis. Additionally, it serves as a critical tool for developing diagnostic assays, therapeutic antibodies, or inhibitors targeting CD177-related disorders. Studies have also explored its potential role in cancer, as CD177 expression is reported in certain malignancies, though its oncogenic mechanisms remain unclear. The availability of recombinant CD177 facilitates mechanistic studies, drug screening, and biomarker discovery, advancing our understanding of its dual roles in immune regulation and disease progression.
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