| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PVRL3 |
| Uniprot No | Q9NQS3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 58-400aa |
| 氨基酸序列 | PI IVEPHVTAVW GKNVSLKCLI EVNETITQIS WEKIHGKSSQ TVAVHHPQYG FSVQGEYQGR VLFKNYSLND ATITLHNIGF SDSGKYICKA VTFPLGNAQS STTVTVLVEP TVSLIKGPDS LIDGGNETVA AICIAATGKP VAHIDWEGDL GEMESTTTSF PNETATIISQ YKLFPTRFAR GRRITCVVKH PALEKDIRYS FILDIQYAPE VSVTGYDGNW FVGRKGVNLK CNADANPPPF KSVWSRLDGQ WPDGLLASDN TLHFVHPLTF NYSGVYICKV TNSLGQRSDQ KVIYISDPPT TTTLQPTIQW HPSTADIEDL ATEPKKLPFP LSTLATIKDD P KSCDKTHTCP PCPAPELLGG PSVFLFPPKP KDTLMISRTP EVTCVVVDVS HEDPEVKFNW YVDGVEVHNA KTKPREEQYN STYRVVSVLT VLHQDWLNGK EYKCKVSNKA LPAPIEKTIS KAKGQPREPQ VYTLPPSRDE LTKNQVSLTC LVKGFYPSDI AVEWESNGQP ENNYKTTPPV LDSDGSFFLY SKLTVDKSRW QQGNVFSCSV MHEALHNHYT QKSLSLSPGK |
| 预测分子量 | 64 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PVRL3重组蛋白的虚构参考文献示例，内容基于典型研究方向设计：
---
1. **文献名称**：*Expression and Purification of Recombinant PVRL3 in Escherichia coli for Structural Studies*
**作者**：Smith J., et al.
**摘要**：本研究报道了利用大肠杆菌系统高效表达PVRL3重组蛋白的优化策略，通过亲和层析和尺寸排阻色谱纯化获得高纯度蛋白，并验证其可用于后续X射线晶体学分析。
2. **文献名称**：*Structural Insights into PVRL3-Mediated Cell Adhesion by Cryo-EM*
**作者**：Chen L., et al.
**摘要**：通过冷冻电镜解析PVRL3胞外域的三维结构，揭示了其与配体nectin-1的相互作用界面，为理解其在细胞间黏附和病毒感染中的双重功能提供了结构基础。
3. **文献名称**：*PVRL3 as a Novel Immune Checkpoint: Role in T-Cell Regulation*
**作者**：Martinez R., et al.
**摘要**：功能研究表明，重组PVRL3蛋白可抑制T细胞活化并调控细胞因子分泌，提示其可能作为肿瘤免疫治疗的新靶点，并通过体外实验验证了这一机制。
4. **文献名称**：*Development of a PVRL3-Based Subunit Vaccine Against Herpesvirus Infection*
**作者**：Kim H., et al.
**摘要**：利用哺乳动物细胞表达的PVRL3重组蛋白作为疫苗抗原，在小鼠模型中诱导了中和抗体并显著降低疱疹病毒载量，证明了其作为预防性疫苗的潜力。
---
注：以上文献为模拟内容，实际研究中请依据真实数据库(如PubMed)检索。
**Background of PVRL3 Recombinant Protein**
PVRL3 (Poliovirus Receptor-Related 3), also known as Nectin-3. is a member of the Nectin family of cell adhesion molecules that play critical roles in mediating cell-cell junctions and tissue organization. Structurally, PVRL3 is a transmembrane glycoprotein containing three extracellular immunoglobulin (Ig)-like domains, which facilitate homophilic or heterophilic interactions with other Nectin family members, such as Nectin-1 (PVRL1) or Nectin-2 (PVRL2). These interactions are essential for forming adherens junctions and tight junctions, particularly in epithelial and neuronal tissues. PVRL3 is also implicated in cellular signaling pathways, often collaborating with cadherins to regulate cell polarity, proliferation, and differentiation.
Recombinant PVRL3 protein is engineered using expression systems (e.g., mammalian cells or *E. coli*) to produce purified, bioactive forms of the protein for research and therapeutic applications. Its recombinant version retains functional extracellular domains, enabling studies on intercellular adhesion mechanisms, viral pathogenesis (e.g., herpesviruses exploit Nectins as entry receptors), and cancer biology, where disrupted cell adhesion often drives metastasis.
In neuroscience, PVRL3’s role in synapse formation and plasticity has garnered interest, while in reproductive biology, it contributes to sperm-egg binding. Additionally, recombinant PVRL3 serves as a tool for developing antiviral agents or therapies targeting cell adhesion abnormalities in diseases like cancer or autoimmune disorders. Its study continues to uncover insights into tissue integrity, disease mechanisms, and potential therapeutic targets.
×
