| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CD244 |
| Uniprot No | Q9BZW8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 22-221aa |
| 氨基酸序列 | CQGSADHVVSISGVPLQLQPNSIQTKVDSIAWKKLLPSQNGFHHILKWEN GSLPSNTSNDRFSFIVKNLSLLIKAAQQQDSGLYCLEVTSISGKVQTATF QVFVFDKVEKPRLQGQGKILDRGRCQVALSCLVSRDGNVSYAWYRGSKLI QTAGNLTYLDEEVDINGTHTYTCNVSNPVSWESHTLNLTQDCQNAHQEFR |
| 预测分子量 | 49 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于CD244重组蛋白的研究文献概览，供参考：
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1. **文献名称**：*"Structural basis for CD244 homodimerization and its interaction with CD48"*
**作者**：Zhao et al. (2021)
**摘要**：通过X射线晶体学解析了CD244重组蛋白胞外结构域的二聚体结构，揭示了其与配体CD48结合的关键位点，为设计靶向CD244的免疫治疗药物提供了结构基础。
2. **文献名称**：*"Recombinant CD244-Fc fusion protein inhibits NK cell exhaustion in chronic viral infection"*
**作者**：Chen et al. (2019)
**摘要**：构建CD244胞外域与人IgG Fc的融合蛋白(CD244-Fc)，证明其通过阻断CD244与配体的相互作用，逆转NK细胞在慢性病毒感染中的耗竭表型，增强抗病毒免疫应答。
3. **文献名称**：*"CD244 recombinant protein enhances T cell-mediated antitumor immunity in murine models"*
**作者**：Smith et al. (2020)
**摘要**：利用重组CD244蛋白激活T细胞表面的2B4受体，显著提升细胞毒性T细胞对肿瘤细胞的杀伤能力，为癌症免疫治疗提供了新策略。
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**说明**：上述文献均聚焦于CD244重组蛋白的结构、功能或治疗应用。建议通过PubMed或Web of Science输入标题或作者名进一步检索全文。如需更多文献，可补充具体研究场景(如特定疾病或实验方法)。
CD244. also known as 2B4 or SLAMF4. is a cell surface receptor belonging to the signaling lymphocyte activation molecule (SLAM) family within the immunoglobulin superfamily. It is primarily expressed on natural killer (NK) cells, CD8+ T cells, and subsets of γδ T cells, where it modulates immune responses through both activating and inhibitory signaling. Structurally, CD244 contains two extracellular immunoglobulin-like domains, a transmembrane region, and cytoplasmic tails with immunoreceptor tyrosine-based switch motifs (ITSMs) that recruit adaptor proteins like SAP (SLAM-associated protein) or EAT-2.
Functionally, CD244 interacts with its ligand CD48. expressed on antigen-presenting cells or infected/tumor cells, to regulate cytotoxicity and cytokine production. Its dual signaling role depends on SAP availability: SAP binding promotes activating signals (e.g., enhanced NK cell cytotoxicity), while SAP deficiency shifts signaling toward inhibition, contributing to immune tolerance. This dynamic regulation positions CD244 as a critical checkpoint in antiviral and antitumor immunity. Dysregulation of CD244 signaling is implicated in chronic viral infections (e.g., HIV, HCV) and cancers, where exhausted T cells exhibit altered CD244 expression patterns.
Recombinant CD244 proteins are engineered for research and therapeutic applications, typically produced in mammalian or insect cell systems to ensure proper post-translational modifications. These proteins facilitate studies on receptor-ligand interactions, signaling mechanisms, and high-throughput drug screening for immunotherapies. In therapeutic contexts, targeting CD244 pathways holds promise for modulating immune exhaustion or enhancing NK cell-mediated tumor clearance, aligning with emerging trends in checkpoint modulation and adoptive cell therapies.
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