| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CD320 |
| Uniprot No | Q9NPF0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 36-231aa |
| 氨基酸序列 | SPLSTPTSAQAAGPSSGSCPPTKFQCRTSGLCVPLTWRCDRDLDCSDGSD EEECRIEPCTQKGQCPPPPGLPCPCTGVSDCSGGTDKKLRNCSRLACLAG ELRCTLSDDCIPLTWRCDGHPDCPDSSDELGCGTNEILPEGDATTMGPPV TLESVTSLRNATTMGPPVTLESVPSVGNATSSSAGDQSGSPTAYGVVDDI EGRMDEPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVT CVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLH QDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSREEMTK NQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKL TVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK |
| 预测分子量 | 47 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CD320重组蛋白的3篇参考文献及其简要摘要：
1. **文献名称**: *"Expression and Functional Characterization of Recombinant CD320 in Vitamin B12 Transport"*
**作者**: Smith A, et al.
**摘要**: 该研究通过哺乳动物细胞表达系统成功制备了CD320重组蛋白，验证其与转钴胺素II(TCN2)的结合能力，并证明其在细胞摄取维生素B12中的关键作用。
2. **文献名称**: *"Structural Insights into CD320 Receptor by Recombinant Protein Crystallography"*
**作者**: Li J, et al.
**摘要**: 利用重组CD320蛋白进行X射线晶体学研究，解析了其三维结构，揭示了其与维生素B12结合的活性位点，为相关代谢疾病的治疗提供结构基础。
3. **文献名称**: *"CD320 Knockout Model and Recombinant Protein Rescue in Cellular B12 Deficiency"*
**作者**: Garcia R, et al.
**摘要**: 通过CRISPR构建CD320基因敲除细胞模型，发现B12吸收缺陷;外源添加重组CD320蛋白可恢复细胞摄取功能，证实其生理必要性。
注：以上文献信息为示例性质，实际引用时请核实具体文献内容及发表情况。
CD320 recombinant protein is a biologically engineered form of the CD320 antigen, a cell-surface receptor also known as the transcobalamin receptor (TCblR) or 8D6A antigen. CD320 is a transmembrane protein primarily expressed in tissues such as the liver, placenta, and brain. It plays a critical role in cellular uptake of vitamin B12 (cobalamin) by binding to the transcobalamin-vitamin B12 complex, facilitating its internalization via endocytosis. This process is essential for maintaining intracellular B12 levels, which are vital for DNA synthesis, red blood cell formation, and neurological function.
Structurally, CD320 contains a large extracellular domain with leucine-rich repeats (LRRs) involved in ligand binding, followed by a transmembrane region and a short cytoplasmic tail. Dysregulation of CD320 has been linked to disorders related to vitamin B12 metabolism, including megaloblastic anemia and neurodegenerative conditions. Its role in cancer biology is also under investigation, as some tumors overexpress CD320 to meet increased metabolic demands.
Recombinant CD320 protein is typically produced in mammalian expression systems (e.g., HEK293 or CHO cells) to ensure proper post-translational modifications. It is purified for use in functional studies, antibody development, or diagnostic assays. Researchers employ it to explore CD320-ligand interactions, screen therapeutic inhibitors, or develop biomarkers for diseases associated with B12 transport defects. Its recombinant form provides a standardized tool to study receptor biology and potential clinical applications in hematology, oncology, and neurology.
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