| 纯度 | > 95 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | BDH1 |
| Uniprot No | Q02338 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-343aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMLATRLSRPLSRLPGKTLSACDRENGARRP LLLGSTSFIPIGRRTYASAAEPVGSKAVLVTGCDSGFGFALAKHLHSKGF LVFAGCLMKDKGHDGVKELDSLNSDRLRTVQLNVCSSEEVEKVVEIVRSS LKDPEKGMWGLVNNAGISTFGEVEFTSLETYKQVAEVNLWGTVRMTKSFL PLIRRAKGRVVNISSMLGRMANPARSPYCITKFGVEAFSDCLRYEMYPLG VKVSVVEPGNFIAATSLYSPESIQAIAKKMWEELPEVVRKDYGKKYFDEK IAKMETYCSSGSTDTSPVIDAVTHALTATTPYTRYHPMDYYWWLRMQIMT HLPGAISDMIYIR |
| 预测分子量 | 40 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于BDH1重组蛋白的3篇参考文献摘要归纳(文献为虚构示例，仅供参考)：
1. **标题**: "Cloning, expression, and characterization of recombinant human BDH1 in Escherichia coli"
**作者**: Zhang Y, et al.
**摘要**: 研究报道了人源BDH1基因在大肠杆菌中的重组表达及纯化方法，验证了其催化β-羟基丁酸与乙酰乙酸相互转化的酶活性，并分析了其最适反应pH与温度条件。
2. **标题**: "Structural insights into the catalytic mechanism of BDH1 through X-ray crystallography"
**作者**: Li H, et al.
**摘要**: 通过X射线晶体学解析了BDH1重组蛋白的三维结构，揭示了其底物结合位点及辅酶NAD+的相互作用机制，为开发代谢疾病相关抑制剂提供结构基础。
3. **标题**: "BDH1-mediated ketone metabolism promotes cancer cell proliferation under glucose deprivation"
**作者**: Wang X, et al.
**摘要**: 研究发现重组BDH1在肿瘤细胞中通过调控酮体代谢途径，增强细胞在低糖微环境下的存活能力，提示BDH1可能成为癌症代谢治疗的潜在靶点。
注：以上文献为模拟内容，实际研究中请通过PubMed、Web of Science等数据库检索真实文献。
BDH1 (β-hydroxybutyrate dehydrogenase 1) is a mitochondrial enzyme encoded by the BDH1 gene, primarily involved in ketone body metabolism. It catalyzes the reversible conversion of β-hydroxybutyrate (a ketone body) to acetoacetate, a critical reaction in energy homeostasis during fasting or metabolic stress. This NAD+/NADH-dependent enzyme plays a key role in balancing hepatic ketogenesis and peripheral tissue utilization of ketones, particularly in the brain, heart, and kidneys during glucose scarcity.
Recombinant BDH1 protein is produced through genetic engineering techniques, typically by cloning the human BDH1 gene into expression vectors (e.g., bacterial, insect, or mammalian systems) followed by purification. Its production enables detailed study of enzymatic kinetics, substrate specificity, and regulatory mechanisms. Researchers utilize recombinant BDH1 to investigate metabolic disorders such as diabetes, obesity, and ketolytic defects, as well as its potential links to neurological conditions and cancer metabolism. Structural studies using recombinant protein have helped map catalytic domains and identify potential drug-binding sites. Current applications also include developing diagnostic tools for metabolic panels and screening compounds that modulate ketone metabolism. The recombinant form preserves the native enzyme's dimeric structure and functional characteristics, making it valuable for both basic research and therapeutic exploration in metabolic diseases.
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