关键信息
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基因名
KRT1
- 应用
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别名
67 kDa cytokeratin Cytokeratin-1 Short name: CK-1 Hair alpha protein Keratin-1 Short name: K1 Type-II keratin Kb1 KRTA
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种属
Human
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表达系统
E. coli
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标签
N- His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P04264
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表达区间
2-644aa
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分子量
69.9 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
KRT1, or Keratin 1, is a type of intermediate filament protein that plays a crucial role in maintaining the structural integrity of keratinocytes and the overall barrier function of the epidermis. It is predominantly expressed in the upper layers of the skin and is essential for skin health and resilience. Mutations or dysregulation of KRT1 have been associated with various skin disorders, particularly epidermolytic hyperkeratosis, which is characterized by severe skin fragility and blistering. Research on recombinant KRT1 proteins has gained traction as scientists seek to understand the mechanisms underlying skin diseases and to explore potential therapeutic interventions. By producing recombinant KRT1 in vitro, researchers can study its functional properties, interactions with other cellular proteins, and its role in skin physiology. Additionally, recombinant KRT1 has potential applications in tissue engineering and regenerative medicine, where it could be used to develop skin substitutes or enhance wound healing. Overall, the study of KRT1 and its recombinant forms is vital for advancing our knowledge of keratin biology and developing strategies for treating skin disorders.
