关键信息
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基因名
MT-CO1
- 应用
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别名
Cytochrome c oxidase polypeptide I (COI) (COXI) (MTCO1)
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种属
Human
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表达系统
E. coli
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标签
N- His-GST
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P00395
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表达区间
474-513aa
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分子量
36.3 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
MT-CO1, or mitochondrial cytochrome c oxidase subunit 1, is a crucial protein involved in the mitochondrial respiratory chain, playing a pivotal role in cellular energy production via oxidative phosphorylation. As part of Complex IV, MT-CO1 catalyzes the transfer of electrons from cytochrome c to molecular oxygen, a process essential for ATP synthesis and cellular respiration. Research on MT-CO1 is significant due to its implications in various fields, including genetics, molecular biology, and bioenergetics. Mutations or dysfunctions in the gene encoding this protein are associated with several mitochondrial diseases, leading to conditions such as Leigh syndrome, which highlights the importance of understanding its structure and function. Additionally, studying MT-CO1 can provide insights into the evolutionary history of eukaryotic organisms, as its sequence is frequently used in phylogenetic analyses. The recombinant production of MT-CO1 allows for in-depth biochemical characterization and structural studies, facilitating the exploration of its functional mechanisms and interactions with other mitochondrial components. Furthermore, insights gained from MT-CO1 research may lead to potential therapeutic strategies for mitochondrial disorders and enhance our understanding of aging and metabolic diseases. Overall, the study of MT-CO1 and its recombinant forms holds substantial promise for advancing knowledge in bioenergetics and developing innovative medical therapies.
