Cat: IPD-X40180

Recombinant Clostridium acetobutylicum ctfA Protein ,His & Myc

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关键信息

  • 基因名

    ctfA

  • 应用

    SPRMSTBLIITCELISA细胞实验药物筛选

  • 别名

    Acetoacetyl-CoA:acetate/butyrate:CoA transferase subunit A (Coat A)

  • 种属

    Clostridium acetobutylicum

  • 表达系统

    E. coli

  • 标签

    N- His & C- Myc

  • 纯度

    Greater than 90% as determined by SDS-PAGE.

  • 蛋白编号

    P33752

  • 表达区间

    1-218aa

  • 分子量

    30.6 kDa

  • 内毒素

    < 1.0 EU per μg protein as determined by the LAL method.

  • 性状

    Freeze-dried powder

  • 缓冲液

    PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

  • 复溶方法

    Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

  • 个性化定制

    点位突变 标签定制 buffer定制 全长蛋白定制

  • 稳定性测试

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.

  • 保存条件 & 期限

    Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

  • 运输条件

    In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.

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背景信息

The research on CTF-A recombinant protein is situated at the intersection of molecular biology and biotechnology, with significant implications in understanding cellular processes and developing therapeutic applications. CTF-A, or Chromatin-Dependent Transcription Factor A, plays a crucial role in regulating gene expression through its interaction with chromatin and transcription machinery. Its function is vital in various biological processes, including cell differentiation and response to stress. Given its importance, scientists have focused on generating recombinant forms of CTF-A to study its structure-function relationships and to elucidate its mechanisms of action at a molecular level. The production of CTF-A as a recombinant protein allows for in-depth analysis of its biochemical properties and interactions with other cellular components. This research is particularly relevant in the context of diseases where transcriptional regulation is disrupted, such as cancer and genetic disorders. By harnessing recombinant DNA technology, researchers can manipulate the CTF-A protein for various experimental designs, aiding in the development of targeted therapies and diagnostic tools. Furthermore, understanding CTF-A's role can provide insights into epigenetic regulation and its implications in developmental biology and disease progression, thus underscoring the significance of this research in advancing both basic science and clinical applications.

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