关键信息
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基因名
cry2Aa
- 应用
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别名
(71 kDa crystal protein)(Crystaline entomocidal protoxin)(Insecticidal delta-endotoxin CryIIA(a))(Mosquito factor)(P2 crystal protein)
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种属
Bacillus thuringiensis subsp. Kurstaki
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表达系统
E. coli
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标签
N- His & C- Myc
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P0A377
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表达区间
267-472aa
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分子量
29.7 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
Cry2Aa is a protein derived from the bacterium *Bacillus thuringiensis* (Bt), known for its insecticidal properties against a range of agricultural pests. As an important member of the Cry protein family, Cry2Aa has garnered significant attention in the fields of biotechnology and entomology due to its potential applications in biocontrol strategies and the development of genetically modified crops. Research on Cry2Aa focuses on its mechanism of action, which involves binding to specific receptors in the midgut of target insects, leading to cell lysis and ultimately, insect mortality. Additionally, studies have been conducted to assess its efficacy against various pest species, evaluate its safety profile for non-target organisms, and understand its environmental impact. The increasing concern regarding chemical pesticides' adverse effects on ecosystems and human health has further driven research into Cry2Aa as a sustainable alternative. Moreover, advancements in molecular biology techniques have enabled scientists to explore methods to enhance the expression and stability of Cry2Aa in transgenic plants, improving their resistance against pests. Overall, the ongoing research on Cry2Aa not only aims to unlock its full potential for agricultural applications but also contributes to the broader field of biological pest control and sustainable agriculture practices.
