关键信息
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基因名
EAAT5
- 应用
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别名
EAAT5；EAAT5；Excitatory amino acid transporter 5
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
O00341
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表达区间
1-560aa
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氨基酸序列
MVPHAILARGRDVCRRNGLLILSVLSVIVGCLLGFFLRTRRLSPQEISYFQFPGELLMRMLKMMILPLVVSSLMSGLASLDAKTSSRLGVLTVAYYLWTTFMAVIVGIFMVSIIHPGSAAQKETTEQSGKPIMSSADALLDLIRNMFPANLVEATFKQYRTKTTPVVKSPKVAPEEAPPRRILIYGVQEENGSHVQNFALDLTPPPEVVYKSEPGTSDGMNVLGIVFFSATMGIMLGRMGDSGAPLVSFCQCLNESVMKIVAVAVWYFPFGIVFLIAGKILEMDDPRAVGKKLGFYSVTVVCGLVLHGLFILPLLYFFITKKNPIVFIRGILQALLIALATSSSSATLPITFKCLLENNHIDRRIARFVLPVGATINMDGTALYEAVAAIFIAQVNNYELDFGQIITISITATAASIGAAGIPQAGLVTMVIVLTSVGLPTDDITLIIAVDWALDRFRTMINVLGDALAAGIMAHICRKDFARDTGTEKLLPCETKPVSLQEIVAAQQNGCVKSVAEASELTLGPTCPHHVPVQVEQDEELPAASLNHCTIQISELETNV
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分子量
60.6 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
EAAT5 (excitatory amino acid transporter 5) is a member of the sodium-dependent glutamate transporter family, primarily expressed in the retina, where it plays a crucial role in the regulation of glutamate levels in the synaptic cleft. This transporter is vital for visual signaling, as it helps to terminate glutamate neurotransmission and prevents excitotoxicity, which can lead to neurodegenerative conditions. Research into the recombinant protein of EAAT5 aims to elucidate its structure-function relationship, kinetics, and regulatory mechanisms, essential for understanding its role in phototransduction and potential involvement in retinal pathologies. Additionally, investigating the recombinant form of EAAT5 allows for the assessment of its interaction with pharmacological agents, which could lead to the development of therapeutic strategies for conditions such as retinal degeneration, epilepsy, and schizophrenia, where glutamate signaling is disrupted. By analyzing the EAAT5 protein through various biochemical and biophysical methods, researchers hope to provide insights into its mechanism of action, paving the way for novel treatments that target glutamate homeostasis in the retina and other regions of the nervous system.
