Cat: PA1000-102DB

Recombinant Human AK4 Protein,His

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关键信息

  • 基因名

    AK4

  • 应用

    SPRMSTBLIITCELISA细胞实验药物筛选

  • 别名

    AK4;GCN2;KIAA1338;eIF-2-alpha kinase GCN2

  • 种属

    Human

  • 表达系统

    E. coli

  • 标签

    His tag N-Terminus

  • 纯度

    Greater than 90% as determined by SDS-PAGE.

  • 蛋白编号

    P27144

  • 表达区间

    1-223aa

  • 氨基酸序列

    MASKLLRAVILGPPGSGKGTVCQRIAQNFGLQHLSSGHFLRENIKASTEVGEMAKQYIEKSLLVPDHVITRLMMSELENRRGQHWLLDGFPRTLGQAEALDKICEVDLVISLNIPFETLKDRLSRRWIHPPSGRVYNLDFNPPHVHGIDDVTGEPLVQQEDDKPEAVAARLRQYKDVAKPVIELYKSRGVLHQFSGTETNKIWPYVYTLFSNKITPIQSKEAY

  • 分子量

    27.8 kDa

  • 内毒素

    < 1.0 EU per μg protein as determined by the LAL method.

  • 性状

    Freeze-dried powder

  • 缓冲液

    PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

  • 复溶方法

    Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

  • 个性化定制

    点位突变 标签定制 buffer定制 全长蛋白定制

  • 稳定性测试

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.

  • 保存条件 & 期限

    Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

  • 运输条件

    In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.

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背景信息

AK4, or adenylate kinase 4, is a member of the adenylate kinase family, which plays a crucial role in cellular energy homeostasis by catalyzing the interconversion of adenine nucleotides. It is primarily localized in mitochondria and is involved in maintaining ATP levels, particularly under conditions of cellular stress or in highly energetic tissues. Research has shown that AK4 may have implications in various physiological and pathological processes, including metabolic disorders and cancer, where altered energy metabolism is a hallmark. The study of AK4's structure and function is vital for understanding its role in mitochondrial dynamics and energy regulation. Moreover, due to its association with disease states, AK4 has become a potential biomarker and therapeutic target. Recent advancements in recombinant protein technology have facilitated the production of AK4 in its purified form, allowing for in-depth biochemical characterization and investigation of its enzymatic activities. Furthermore, the study of AK4 reconstitution plays a pivotal role in elucidating its mechanisms and potential interactions with other mitochondrial proteins, providing insights into its contribution to cellular function and dysfunction. Overall, ongoing research aims to decode the precise molecular functions of AK4, exploring its relevance in health and disease, and identifying potential strategies for targeting this enzyme in therapeutic interventions.

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