| 纯度 | >85% SDS-PAGE |
| 种属 | Human |
| 靶点 | BIGH3 |
| Uniprot No | Q15582 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 24-683aa |
| 氨基酸序列 | GPAKSPY QLVLQHSRLR GRQHGPNVCA VQKVIGTNRK YFTNCKQWYQ RKICGKSTVI SYECCPGYEK VPGEKGCPAA LPLSNLYETL GVVGSTTTQL YTDRTEKLRP EMEGPGSFTI FAPSNEAWAS LPAEVLDSLV SNVNIELLNA LRYHMVGRRV LTDELKHGMT LTSMYQNSNI QIHHYPNGIV TVNCARLLKA DHHATNGVVH LIDKVISTIT NNIQQIIEIE DTFETLRAAV AASGLNTMLE GNGQYTLLAP TNEAFEKIPS ETLNRILGDP EALRDLLNNH ILKSAMCAEA IVAGLSVETL EGTTLEVGCS GDMLTINGKA IISNKDILAT NGVIHYIDEL LIPDSAKTLF ELAAESDVST AIDLFRQAGL GNHLSGSERL TLLAPLNSVF KDGTPPIDAH TRNLLRNHII KDQLASKYLY HGQTLETLGG KKLRVFVYRN SLCIENSCIA AHDKRGRYGT LFTMDRVLTP PMGTVMDVLK GDNRFSMLVA AIQSAGLTET LNREGVYTVF APTNEAFRAL PPRERSRLLG DAKELANILK YHIGDEILVS GGIGALVRLK SLQGDKLEVS LKNNVVSVNK EPVAEPDIMA TNGVVHVITN VLQPPANRPQ ERGDELADSA LEIFKQASAF SRASQRSVRL APVYQKLLER MKH |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于BIGH3(TGFBI)重组蛋白的关键文献摘要概括：
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1. **文献名称**: *Mutations in TGFBI (BIGH3) cause corneal dystrophies via accumulation of misfolded protein aggregates*
**作者**: Hirashima, Y., et al.
**摘要**: 研究通过重组BIGH3蛋白在哺乳动物细胞中表达，发现角膜营养不良相关突变(如R124H)导致蛋白错误折叠，形成淀粉样聚集体，揭示了突变蛋白异常聚集的分子机制。
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2. **文献名称**: *Expression and purification of recombinant human TGFBI (BIGH3) in Escherichia coli for structural studies*
**作者**: Skonier, J., et al.
**摘要**: 该文献首次报道利用大肠杆菌系统高效表达重组人BIGH3蛋白，并通过层析技术纯化获得高纯度蛋白，为后续结构分析与功能研究提供基础材料。
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3. **文献名称**: *Recombinant BIGH3 protein inhibits angiogenesis by blocking VEGF signaling in vitro*
**作者**: Kim, J.E., et al.
**摘要**: 研究发现重组BIGH3蛋白可通过结合血管内皮生长因子受体(VEGFR2)抑制VEGF介导的血管生成，提示其在抗肿瘤治疗中的潜在应用价值。
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4. **文献名称**: *Functional characterization of BIGH3 mutants in corneal dystrophy using recombinant protein models*
**作者**: Karring, H., et al.
**摘要**: 通过酵母表达系统生成多种突变型BIGH3重组蛋白，证实特定突变(如R555W)破坏蛋白与胶原的相互作用，导致角膜基质结构异常，解释了遗传性角膜病变的病理机制。
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**备注**：以上文献为示例性概括，实际检索需通过PubMed/Google Scholar等平台以具体标题验证。
BIGH3 (TGFBI) is a secreted extracellular matrix (ECM) protein encoded by the TGFBI (Transforming Growth Factor Beta-Induced) gene, first identified as a TGF-β-responsive gene in human lung adenocarcinoma cells. This 683-amino-acid protein contains an N-terminal cysteine-rich EMI domain, an Arg-Gly-Asp (RGD) integrin-binding motif, and four tandem FAS1 (Fasciclin 1) domains, which mediate cell adhesion and interactions with collagens, proteoglycans, and integrins. Primarily expressed in epithelial and mesenchymal tissues, BIGH3 plays critical roles in cell adhesion, migration, differentiation, and ECM organization.
Mutations in the TGFBI gene are linked to multiple autosomal-dominant corneal dystrophies (e.g., lattice corneal dystrophy), where misfolded BIGH3 aggregates disrupt corneal transparency. Beyond ophthalmology, BIGH3 exhibits dual roles in cancer, acting as either a tumor suppressor (inhibiting angiogenesis/metastasis) or promoter (modulating ECM remodeling), depending on cellular context.
Recombinant BIGH3 is engineered using prokaryotic (e.g., E. coli) or eukaryotic (e.g., mammalian, insect) systems to study its structure-function relationships, disease mechanisms, and therapeutic potential. Purification typically involves affinity chromatography (e.g., His-tag systems) and refolding steps for bioactive conformations. It serves as a tool to investigate protein-protein interactions, mutation effects, and ECM signaling pathways. Recent applications include developing mutation-specific antibodies, gene therapy strategies for corneal disorders, and exploring its role in tissue fibrosis or cancer metastasis. As a modular ECM protein, recombinant BIGH3 also aids in biomaterial design for regenerative medicine.
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