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Rabbit Polyclonal HIST2H2AB(N-term) Antibody

  • 中文名： HIST2H2AB (N-term)抗体
  • 别    名： Histone H2A type 2-B, HIST2H2AB
货号: IPDX31327
Price： ￥1180
数量：
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验证与应用

应用及物种
WB 1/1000 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 咨询技术 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesPHN; CPSASE1
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman, Mouse, Rat
ImmunogenSynthetic peptide of human CPS1
FormulationPurified antibody in PBS with 0.05% sodium azide and 50% glycerol.

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参考文献

以下是关于HIST2H2AB(N-term)抗体的3篇参考文献，涵盖其在不同研究中的应用及发现：

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1. **文献名称**：*"Site-specific phosphorylation dynamics of the human histone H2A family during the cell cycle"*

**作者**：Sugiyama N. et al.

**摘要**：本研究通过质谱分析和HIST2H2AB(N-term)特异性抗体，揭示了H2A家族成员(包括HIST2H2AB)在细胞周期不同阶段的磷酸化位点动态变化，表明其N端修饰可能参与染色质重塑和DNA损伤修复。

2. **文献名称**：*"Epigenetic regulation of pluripotency by histone H2A variants in embryonic stem cells"*

**作者**：Shinagawa T. et al.

**摘要**：利用HIST2H2AB(N-term)抗体进行染色质免疫沉淀(ChIP-seq)，发现HIST2H2AB在胚胎干细胞多能性基因启动子区域富集，提示其通过表观遗传机制调控干细胞自我更新。

3. **文献名称**：*"Antibody-based profiling of histone post-translational modifications in cancer"*

**作者**：Beck D.B. et al.

**摘要**：评估了多种组蛋白修饰抗体的特异性，包括HIST2H2AB(N-term)抗体，证实其在肿瘤样本中检测H2A变体修饰的可靠性，并发现HIST2H2AB异常修饰与结直肠癌进展相关。

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**备注**：若需获取全文，建议通过PubMed或Google Scholar搜索文献标题及作者，部分研究可通过开放获取平台(如Nature Communications、Cell Reports)下载。实际应用中，也可参考抗体供应商(如CST、Abcam)提供的技术文档及引用文献。

背景信息

The HIST2H2AB (N-term) antibody specifically targets the N-terminal region of histone H2A type 2-B (HIST2H2AB), a core component of nucleosomes. Histones, including H2A, play essential roles in chromatin structure and epigenetic regulation by compacting DNA into higher-order structures and undergoing post-translational modifications (e.g., acetylation, methylation) that influence gene expression. The HIST2H2AB gene, located on chromosome 1q42.13. encodes a replication-dependent histone variant involved in DNA packaging, repair, and transcriptional regulation. Antibodies targeting the N-terminal region are valuable for studying histone modifications, chromatin dynamics, and their roles in cellular processes like cell cycle progression, apoptosis, and DNA damage response. These antibodies are commonly used in techniques such as Western blotting, chromatin immunoprecipitation (ChIP), and immunofluorescence to investigate histone localization, modification states, or interactions with chromatin-associated proteins. Specificity for the N-terminus ensures recognition of epitopes that may be critical for modification-dependent interactions. Researchers often validate such antibodies using knockout controls or peptide-blocking assays to confirm target specificity, especially given the high sequence homology among histone family members. Applications span cancer biology, developmental studies, and epigenetic research, where dysregulation of histone H2A variants is linked to diseases like malignancies and genetic disorders.

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