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Rabbit Polyclonal MousePrkca(N-term) Antibody

  • 中文名： Mouse Prkca (N-term)抗体
  • 别    名： Protein kinase C alpha type, PKC-A, PKC-alpha, Prkca, Pkca
货号: IPDX31434
Price： ￥1180
数量：
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验证与应用

应用及物种
WB 1/1000 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 1/100-1/500 Human,Mouse,Rat
ICC 1/10-1/50 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesProtein kinase C alpha type, PKC-A, PKC-alpha, Prkca, Pkca
Entrez GeneID18750
WB Predicted band size76.9kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman, Mouse
ImmunogenThis Mouse Prkca antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of mouse Prkca.
FormulationPurified antibody in PBS with 0.05% sodium azide.

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参考文献

以下是关于Mouse Prkca (N-term)抗体的3篇参考文献示例(注：文献为虚构示例，实际引用需核实)：

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1. **文献名称**：*Role of PKCα in Neuronal Development: Insights from N-terminal Specific Antibodies*

**作者**：Smith J, et al.

**摘要**：研究利用针对小鼠PKCα N端的抗体，通过免疫印迹和免疫荧光技术，揭示了PKCα在小鼠大脑皮层神经元分化中的关键作用，并验证了抗体的特异性。

2. **文献名称**：*PKCα N-terminal Antibody in Cardiac Hypertrophy Models*

**作者**：Lee H, et al.

**摘要**：通过Western blot和免疫组化实验，该文献验证了小鼠PKCα N端抗体在心肌细胞中的特异性，并发现PKCα在压力诱导的心肌肥厚中异常激活。

3. **文献名称**：*Characterization of PKCα Isoform-Specific Signaling in Mouse Macrophages*

**作者**：Garcia R, et al.

**摘要**：研究使用N端特异性抗体分析小鼠巨噬细胞中PKCα的亚细胞定位，证明其在炎症信号通路中的动态分布及功能。

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**说明**：以上文献为示例，实际引用需通过PubMed、Google Scholar等平台搜索关键词(如“PKCα antibody N-terminal mouse”)获取真实文献，并确保抗体应用场景匹配实验需求。

背景信息

The Mouse PRKCA (N-term) antibody is a monoclonal or polyclonal antibody designed to specifically recognize the N-terminal region of protein kinase C alpha (PRKCA) in mouse samples. PRKCA, a member of the classical PKC subfamily, is a serine/threonine kinase critical for regulating diverse cellular processes, including proliferation, differentiation, apoptosis, and signal transduction. Its N-terminal regulatory domain contains motifs like C1 and C2. which mediate lipid binding and membrane translocation upon activation by calcium ions and diacylglycerol (DAG). This antibody is commonly used in techniques such as Western blotting, immunohistochemistry, and immunofluorescence to study PRKCA expression, localization, and post-translational modifications in tissues or cell lines. Researchers employ it to explore PRKCA's role in cancer, neurological disorders, and cardiovascular diseases, where dysregulated PKCα signaling is implicated. Validation typically includes testing in PRKCA-knockout models to confirm specificity. The N-terminal targeting minimizes cross-reactivity with other PKC isoforms (e.g., PKCβ or PKCγ), enhancing reliability in detecting endogenous PKCα. Its applications extend to drug discovery and mechanistic studies, particularly in pathways involving phospholipase C (PLC) and G protein-coupled receptors (GPCRs). Proper controls, such as peptide blocking assays, are recommended to ensure antibody specificity.

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