关键信息
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基因名
CYP1A1
- 应用
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别名
CYP1A1；ARO1；CYAR；CYP19；Aromatase
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P04798
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表达区间
1-512aa
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氨基酸序列
MLFPISMSATEFLLASVIFCLVFWVIRASRPQVPKGLKNPPGPWGWPLIG HMLTLGKNPHLALSRMSQQYGDVLQIRIGSTPVVVLSGLDTIRQALVRQG DDFKGRPDLYTFTLISNGQSMSFSPDSGPVWAARRRLAQNGLKSFSIASD PASSTSCYLEEHVSKEAEVLISTLQELMAGPGHFNPYRYVVVSVTNVICA ICFGRRYDHNHQELLSLVNLNNNFGEVVGSGNPADFIPILRYLPNPSLNA FKDLNEKFYSFMQKMVKEHYKTFEKGHIRDITDSLIEHCQEKQLDENANV QLSDEKIINIVLDLFGAGFDTVTTAISWSLMYLVMNPRVQRKIQEELDTV IGRSRRPRLSDRSHLPYMEAFILETFRHSSFVPFTIPHSTTRDTSLKGFY IPKGRCVFVNQWQINHDQKLWVNPSEFLPERFLTPDGAIDKVLSEKVIIF GMGKRKCIGETIARWEVFLFLAILLQRVEFSVPLGVKVDMTPIYGLTMKH ACCEHFQMQLRS
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分子量
82 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
CYP1A1, a member of the cytochrome P450 enzyme family, plays a crucial role in the metabolism of xenobiotics and endogenous compounds, particularly in the biotransformation of polycyclic aromatic hydrocarbons (PAHs) and other environmental toxins. This enzyme is primarily expressed in the liver and is regulated by various factors, including exposure to environmental pollutants, dietary components, and genetic polymorphisms. Research has demonstrated that CYP1A1 is involved in bioactivation pathways that can lead to the formation of reactive intermediates, contributing to toxicological effects and carcinogenesis. Understanding the structure and function of CYP1A1 through the use of recombinant protein technology has become imperative for elucidating its role in drug metabolism, toxicology, and disease susceptibility. The expression of recombinant CYP1A1 enables the detailed study of its enzymatic properties, substrate specificity, and interaction with various inhibitors, providing insights into its biochemical mechanisms. Moreover, investigating the implications of CYP1A1 activity in the context of individual variances in drug response and disease risk enhances the comprehension of personalized medicine and pharmacogenetics. Therefore, ongoing research into CYP1A1 recombinant protein not only contributes to basic scientific knowledge but also holds significant potential for applications in toxicology, pharmacology, and environmental health.
