关键信息
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基因名
CRLF1
- 应用
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别名
CRLF1；Cytokine receptor-like factor 1
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
O75462
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表达区间
38-422aa
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氨基酸序列
AHTAVISPQDPTLLIGSSLLATCSVHGDPPGATAEGLYWTLNGRRLPPELSRVLNASTLALALANLNGSRQRSGDNLVCHARDGSILAGSCLYVGLPPEKPVNISCWSKNMKDLTCRWTPGAHGETFLHTNYSLKYKLRWYGQDNTCEEYHTVGPHSCHIPKDLALFTPYEIWVEATNRLGSARSDVLTLDILDVVTTDPPPDVHVSRVGGLEDQLSVRWVSPPALKDFLFQAKYQIRYRVEDSVDWKVVDDVSNQTSCRLAGLKPGTVYFVQVRCNPFGIYGSKKAGIWSEWSHPTAASTPRSERPGPGGGACEPRGGEPSSGPVRRELKQFLGWLKKHAYCSNLSFRLYDQWRAWMQKSHKTRNQDEGILPSGRRGTARGPAR
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分子量
46.5 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
CRLF1 (Cytokine Receptor-Like Factor 1) is a member of the IL-6 cytokine receptor family, playing a pivotal role in hematopoiesis and immune responses. Research has increasingly focused on CRLF1 due to its association with various hematological disorders, notably its involvement in the pathogenesis of acute lymphoblastic leukemia (ALL). The emergence of CRLF1 as a critical receptor in mediating signaling pathways has spurred interest in understanding its structure and function through recombinant protein techniques. Producing CRLF1 as a recombinant protein allows scientists to investigate its biochemical properties, receptor interactions, and downstream signaling mechanisms in detail. This research not only elucidates CRLF1's role in normal physiological processes but also provides insights into its potential as a therapeutic target in malignancies associated with its dysregulation. Furthermore, characterizing CRLF1 can facilitate the development of novel diagnostic tools and treatment modalities, enhancing the clinical management of related diseases. Overall, the study of CRLF1 recombinant proteins holds significant promise for advancing our understanding of both normal immune function and the pathophysiological mechanisms underlying associated disorders.
