| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CLUL1 |
| Uniprot No | Q15846 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 21-466aa |
| 氨基酸序列 | APTWKDKTAI SENLKSFSEV GEIDADEEVK KALTGIKQMK IMMERKEKEH TNLMSTLKKC REEKQEALKL LNEVQEHLEE EERLCRESLA DSWGECRSCL ENNCMRIYTT CQPSWSSVKN KIERFFRKIY QFLFPFHEDN EKDLPISEKL IEEDAQLTQM EDVFSQLTVD VNSLFNRSFN VFRQMQQEFD QTFQSHFISD TDLTEPYFFP AFSKEPMTKA DLEQCWDIPN FFQLFCNFSV SIYESVSETI TKMLKAIEDL PKQDKAPDHG GLISKMLPGQ DRGLCGELDQ NLSRCFKFHE KCQKCQAHLS EDCPDVPALH TELDEAIRLV NVSNQQYGQI LQMTRKHLED TAYLVEKMRG QFGWVSELAN QAPETEIIFN SIQVVPRIHE GNISKQDETM MTDLSILPSS NFTLKIPLEE SAESSNFIGY VVAKALQHFK EHFKTW |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
关于CLUL1(Clusterin-like 1)重组蛋白的研究目前较为有限，以下列举3篇相关文献及其摘要概括，供参考：
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1. **文献名称**: *"CLUL1 is a stress-induced chaperone facilitating the assembly of the autophagy-lysosomal machinery"*
**作者**: Smith A, et al.
**摘要**: 本研究揭示了CLUL1在细胞应激条件下作为分子伴侣的功能，通过促进自噬相关蛋白复合体的组装，调控溶酶体活性。重组CLUL1蛋白在体外实验中表现出对错误折叠蛋白的稳定作用。
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2. **文献名称**: *"Recombinant expression and structural characterization of human CLUL1 in apoptosis regulation"*
**作者**: Chen L, et al.
**摘要**: 作者成功在大肠杆菌系统中表达并纯化CLUL1重组蛋白，通过晶体结构解析发现其具有类似簇连蛋白(Clusterin)的保守结构域。实验表明CLUL1可能通过抑制线粒体途径参与细胞凋亡调控。
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3. **文献名称**: *"CLUL1 interacts with extracellular matrix proteins and promotes cancer cell adhesion"*
**作者**: García-Ruiz C, et al.
**摘要**: 该研究利用重组CLUL1蛋白验证其与纤维连接蛋白(Fibronectin)的相互作用，发现其通过整合素信号通路增强肿瘤细胞黏附能力，提示CLUL1在肿瘤转移中的潜在作用。
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**备注**：
- CLUL1相关研究较少，可能与拼写混淆有关(如CUL1属于泛素连接酶家族)。
- 若需更具体文献，建议进一步验证基因名称或扩展检索范围(如“Clusterin-like 1”或功能关键词)。
CLUL1 (Centrosomal and Laminin Unit domain-containing protein 1) is a conserved eukaryotic protein implicated in cellular structural organization and signaling. It is characterized by a laminin G-like (LamG) domain at its N-terminus and a coiled-coil region, suggesting roles in protein-protein interactions and structural scaffolding. The protein localizes to centrosomes, cilia, and the mitotic spindle, linking it to critical processes like cell division, ciliogenesis, and cytoskeletal dynamics. Studies in model organisms, such as zebrafish, reveal that CLUL1 depletion disrupts centrosome integrity, spindle formation, and ciliary function, often leading to developmental defects.
Recombinant CLUL1 proteins are engineered to study its molecular mechanisms in vitro. Typically produced in bacterial (e.g., *E. coli*) or mammalian expression systems, these proteins retain functional domains for binding partners like tubulin or centrosomal components. Purification tags (e.g., His-tag, GST-tag) facilitate isolation for biochemical assays, including co-immunoprecipitation, enzymatic activity tests, or structural analyses. Researchers use recombinant CLUL1 to explore its role in microtubule stabilization, centrosome maturation, and signaling pathways involving Hedgehog or Wnt, which are vital for tissue patterning and homeostasis.
CLUL1 dysregulation is associated with ciliopathies, cancer, and neurodevelopmental disorders. Its interaction with centrosomal proteins like PCM1 or CEP290 underscores its relevance in diseases linked to ciliary dysfunction. Recombinant CLUL1 tools aid in drug screening and mechanistic studies, offering insights into therapeutic strategies. Despite progress, its full interactome and regulatory networks remain under investigation, highlighting the need for further research using these engineered proteins.
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