Cat: PA1000-418DB

Recombinant Human CAPNS1 Protein,His

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关键信息

  • 基因名

    CAPNS1

  • 应用

    SPRMSTBLIITCELISA细胞实验药物筛选

  • 别名

    CAPNS1;CAPN4;CAPNS;Calpain small subunit 1

  • 种属

    Human

  • 表达系统

    E. coli

  • 标签

    His tag N-Terminus

  • 纯度

    Greater than 90% as determined by SDS-PAGE.

  • 蛋白编号

    P04632

  • 表达区间

    84-268aa

  • 氨基酸序列

    MGSSHHHHHH SSGLVPRGSH MGSRTHYSNI EANESEEVRQ FRRLFAQLAG DDMEVSATEL MNILNKVVTR HPDLKTDGFG IDTCRSMVAV MDSDTTGKLG FEEFKYLWNN IKRWQAIYKQ FDTDRSGTIC SSELPGAFEA AGFHLNEHLY NMIIRRYSDE SGNMDFDNFI SCLVRLDAMF RAFKSLDKDG TGQIQVNIQE WLQLTMYS

  • 分子量

    24 kDa

  • 内毒素

    < 1.0 EU per μg protein as determined by the LAL method.

  • 性状

    Freeze-dried powder

  • 缓冲液

    PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

  • 复溶方法

    Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

  • 个性化定制

    点位突变 标签定制 buffer定制 全长蛋白定制

  • 稳定性测试

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.

  • 保存条件 & 期限

    Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

  • 运输条件

    In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.

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背景信息

CAPNS1, or Calpain Small Subunit 1, is a critical protein implicated in various cellular processes, including cell signaling, apoptosis, and cytoskeletal dynamics. Research into CAPNS1 has gained momentum owing to its potential role in several diseases, including cancer and neurological disorders. As a component of the calpain complex, CAPNS1 regulates calpain activity, which is essential for proteolytic processes necessary for normal cellular functions. Dysregulation of calpain activity, often linked to aberrant CAPNS1 functioning, has been associated with tissue remodeling, inflammation, and cell death, making it a focal point for therapeutic interventions. Understanding the structure and function of CAPNS1 through recombinant protein studies could unravel its mechanisms in health and disease, paving the way for novel treatment strategies. Recent studies employing techniques such as X-ray crystallography and site-directed mutagenesis have begun to elucidate the intricate roles of CAPNS1, highlighting its significance in the calpain system's regulation. Given its involvement in critical biological pathways, CAPNS1 is positioned as a promising target for drug design, particularly in conditions where calpain dysregulation plays a pivotal role. Therefore, the characterization and functional studies of CAPNS1 recombinant protein remain fundamental in the context of advancing our understanding of its biological significance and therapeutic potential.

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