关键信息
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基因名
PADI6
- 应用
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别名
PADI6；PAD6；Protein-arginine deiminase type-6
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q6TGC4
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表达区间
1-694aa
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氨基酸序列
MVSVEGRAMS FQSIIHLSLD SPVHAVCVLG TEICLDLSGC APQKCQCFTI HGSGRVLIDV ANTVISEKED ATIWWPLSDP TYATVKMTSP SPSVDADKVS VTYYGPNEDA PVGTAVLYLT GIEVSLEVDI YRNGQVEMSS DKQAKKKWIW GPSGWGAILL VNCNPADVGQ QLEDKKTKKV IFSEEITNLS QMTLNVQGPS CILKKYRLVL HTSKEESKKA RVYWPQKDNS STFELVLGPD QHAYTLALLG NHLKETFYVE AIAFPSAEFS GLISYSVSLV EESQDPSIPE TVLYKDTVVF RVAPCVFIPC TQVPLEVYLC RELQLQGFVD TVTKLSEKSN SQVASVYEDP NRLGRWLQDE MAFCYTQAPH KTTSLILDTP QAADLDEFPM KYSLSPGIGY MIQDTEDHKV ASMDSIGNLM VSPPVKVQGK EYPLGRVLIG SSFYPSAEGR AMSKTLRDFL YAQQVQAPVE LYSDWLMTGH VDEFMCFIPT DDKNEGKKGF LLLLASPSAC YKLFREKQKE GYGDALLFDE LRADQLLSNG REAKTIDQLL ADESLKKQNE YVEKCIHLNR DILKTELGLV EQDIIEIPQL FCLEKLTNIP SDQQPKRSFA RPYFPDLLRM IVMGKNLGIP KPFGPQIKGT CCLEEKICCL LEPLGFKCTF INDFDCYLTE VGDICACANI RRVPFAFKWW KMVP
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
PADI6, a member of the peptidylarginine deiminase (PAD) enzyme family, plays a crucial role in post-translational modification processes, specifically in the citrullination of proteins. This enzyme has garnered significant attention in recent years due to its involvement in various physiological and pathological conditions, including cancer, autoimmune diseases, and reproductive biology. Research indicates that PADI6 is particularly important in oocyte maturation and early embryonic development, where it facilitates the transition from a quiescent state to active development by modifying arginine residues in key proteins. The aberrant regulation of PADI6 has been linked to infertility and developmental disorders, underscoring its biological significance. Moreover, PADI6's potential as a therapeutic target is being explored, with studies aiming to inhibit or modulate its activity for beneficial effects in disease contexts. However, despite extensive investigation into its biological roles, the precise mechanisms by which PADI6 influences cellular pathways and the implications of its dysregulation remain inadequately understood, highlighting the need for further exploration. This ongoing research aims to elucidate the functional mechanisms of PADI6 and its potential as a biomarker or therapeutic target in clinical settings.
