| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PACSIN1 |
| Uniprot No | Q9BY11 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-444aa |
| 氨基酸序列 | MSSSYDEASL APEETTDSFW EVGNYKRTVK RIDDGHRLCN DLMNCVQERA KIEKAYGQQL TDWAKRWRQL IEKGPQYGSL ERAWGAIMTE ADKVSELHQE VKNNLLNEDL EKVKNWQKDA YHKQIMGGFK ETKEAEDGFR KAQKPWAKKM KELEAAKKAY HLACKEEKLA MTREMNSKTE QSVTPEQQKK LQDKVDKCKQ DVQKTQEKYE KVLEDVGKTT PQYMENMEQV FEQCQQFEEK RLVFLKEVLL DIKRHLNLAE NSSYIHVYRE LEQAIRGADA QEDLRWFRST SGPGMPMNWP QFEEWNPDLP HTTTKKEKQP KKAEGVALTN ATGAVESTSQ AGDRGSVSSY DRGQPYATEW SDDESGNPFG GSETNGGANP FEDDSKGVRV RALYDYDGQE QDELSFKAGD ELTKLGEEDE QGWCRGRLDS GQLGLYPANY VEAI |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PACSIN1重组蛋白的3篇参考文献及其摘要：
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1. **文献名称**：*PACSIN 1 forms a complex with dynamin 1 and controls its activity in chromaffin cells*
**作者**：Plomann, M., et al.
**摘要**：该研究利用重组PACSIN1蛋白，揭示了其通过SH3结构域与dynamin 1的相互作用，调控神经内分泌细胞(如嗜铬细胞)中囊泡内吞的动态过程，表明PACSIN1在膜重塑中的关键作用。
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2. **文献名称**：*Regulation of the neuronal endocytic machinery by PACSIN1 through direct binding*
**作者**：Modregger, J., et al.
**摘要**：通过体外重组蛋白实验，作者发现PACSIN1通过结合突触蛋白(如synaptojanin 1)，调控神经元内吞复合体的组装，并影响突触囊泡的回收效率，提示其在神经信号传导中的功能。
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3. **文献名称**：*Tyrosine phosphorylation of PACSIN 1 regulates its function in membrane trafficking*
**作者**：Ritter, B., et al.
**摘要**：本研究利用重组PACSIN1蛋白进行磷酸化分析，发现其酪氨酸残基磷酸化可改变与F-actin及网格蛋白的亲和力，从而调节细胞膜运输和细胞骨架重塑过程。
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**备注**：以上文献为示例，实际引用时建议通过PubMed或Google Scholar核对最新研究。如需补充第4篇，可参考Qualmann, B.等人关于PACSIN1与WASP蛋白协同调控肌动蛋白聚合的工作。
PACSIN1 (Protein kinase C and casein kinase substrate in neurons 1), also known as Syndapin-1. is a member of the PACSIN/Syndapin family of cytoplasmic phosphoproteins involved in membrane dynamics and cytoskeletal organization. It is characterized by an N-terminal F-BAR (FES-CIP4 homology Bin/Amphiphysin/Rvs) domain, which facilitates membrane curvature sensing and binding to phospholipids, and a C-terminal SH3 (Src homology 3) domain that mediates protein-protein interactions. PACSIN1 plays critical roles in vesicle trafficking, endocytosis, and actin remodeling, particularly in neuronal and non-neuronal cells.
Recombinant PACSIN1 protein is engineered for in vitro studies to elucidate its structural and functional properties. It is commonly expressed in bacterial (e.g., *E. coli*) or mammalian systems to ensure proper folding and post-translational modifications. The recombinant form often includes affinity tags (e.g., His-tag, GST) for purification and detection. Researchers utilize this protein to investigate its interactions with binding partners, such as dynamin, N-WASP, and synaptojanin, which are essential for clathrin-mediated endocytosis and actin polymerization.
Studies highlight PACSIN1's involvement in neurological processes, including synaptic vesicle recycling and neurite outgrowth. Dysregulation of PACSIN1 has been linked to neurodevelopmental disorders and cancers, emphasizing its therapeutic potential. Recombinant PACSIN1 enables mechanistic insights into membrane deformation, cytoskeletal dynamics, and disease pathways, serving as a valuable tool for biochemical assays, structural studies, and drug discovery. Its versatility in mimicking native protein functions makes it pivotal for advancing research in cell biology and molecular medicine.
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