关键信息
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基因名
AQP3
- 应用
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别名
AQP3；Aquaporin-3
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q92482
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表达区间
1-292aa
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氨基酸序列
MGRQKELVSRCGEMLHIRYRLLRQALAECLGTLILVMFGCGSVAQVVLSRGTHGGFLTINLAFGFAVTLGILIAGQVSGAHLNPAVTFAMCFLAREPWIKLPIYTLAQTLGAFLGAGIVFGLYYDAIWHFADNQLFVSGPNGTAGIFATYPSGHLDMINGFFDQFIGTASLIVCVLAIVDPYNNPVPRGLEAFTVGLVVLVIGTSMGFNSGYAVNPARDFGPRLFTALAGWGSAVFTTGQHWWWVPIVSPLLGSIAGVFVYQLMIGCHLEQPPPSNEEENVKLAHVKHKEQI
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分子量
31.5 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
Aquaporin-3 (AQP3) is a member of the aquaporin family, which consists of water and solute channels that facilitate the transport of water, glycerol, and other small molecules across the cell membrane. AQP3 is particularly abundant in kidney, skin, and various epithelial tissues, playing a pivotal role in maintaining water homeostasis and regulating osmotic balance. Understanding the structure and function of AQP3 has profound implications for diverse physiological processes, including renal concentration, skin hydration, and cellular metabolism. Research has shown that AQP3 is involved in various pathophysiological conditions, such as dehydration, edema, and even certain cancers, making it a target for therapeutic interventions. The recombinant protein expression of AQP3 allows for detailed biophysical studies and elucidates its functional mechanisms. Purified AQP3 can be studied using techniques such as crystallography and electrophysiology, providing insights into its channel properties and regulatory mechanisms. The ability to produce AQP3 in a laboratory setting also supports drug screening initiatives designed to modulate its activity. Overall, the study of recombinant AQP3 not only enhances our understanding of its biological significance but also opens avenues for innovative treatments related to fluid balance disorders and other AQP3-mediated diseases.
