关键信息
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基因名
CDC34
- 应用
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别名
CDC34；UBCH3；UBE2R1；Ubiquitin-conjugating enzyme E2 R1
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P49427
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表达区间
1-236aa
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氨基酸序列
MARPLVPSSQKALLLELKGLQEEPVEGFRVTLVDEGDLYNWEVAIFGPPN TYYEGGYFKARLKFPIDYPYSPPAFRFLTKMWHPNIYETGDVCISILHPP VDDPQSGELPSERWNPTQNVRTILLSVISLLNEPNTFSPANVDASVMYRK WKESKGKDREYTDIIRKQVLGTKVDAERDGVKVPTTLAEYCVKTKAPAPD EGSDLFYDDYYEDGEVEEEADSCFGDDEDDSGTEES
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分子量
33 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
CDC34 is a crucial component of the ubiquitin-proteasome pathway, which plays a significant role in regulating protein degradation and cell cycle progression. As an E2 ubiquitin-conjugating enzyme, CDC34 facilitates the transfer of ubiquitin moieties to target proteins, marking them for proteasomal degradation. This process is vital for maintaining cellular homeostasis and ensuring proper cellular responses to various stimuli. Abnormalities in the CDC34 function are associated with multiple diseases, including cancer, where dysregulated protein degradation contributes to uncontrolled cell proliferation. The study of CDC34 recombinant proteins is essential for understanding its functional mechanisms and interactions with other cellular components. By generating and analyzing CDC34 in a recombinant form, researchers can investigate its enzymatic activity, binding partners, and structural properties in detail. This research not only sheds light on the molecular underpinnings of CDC34-related pathways but also paves the way for potential therapeutic interventions targeting the ubiquitin-proteasome system in diseases linked to CDC34 dysfunction. Overall, understanding CDC34's role in cellular regulation is critical for elucidating its contributions to health and disease.
