| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TUBB4A |
| Uniprot No | P04350 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-444aa |
| 氨基酸序列 | MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGNYVPRAVLVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDAVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEFPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLSVQSKNSSYFVEWIPNNVKTAVCDIPPRGLKMAATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATAEEGEFEEEAEEEVA |
| 预测分子量 | 53.6kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于 TUBB4A 重组蛋白研究的 **示例参考文献**(部分为虚构，仅作格式参考)：
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1. **文献名称**: *TUBB4A mutations impair microtubule dynamics in a reconstituted in vitro system*
**作者**: Simmons, A. et al.
**摘要**: 研究利用重组 TUBB4A 蛋白在 HEK293 细胞中表达，发现特定突变(如 D249N)显著降低微管聚合速率，并破坏微管网络稳定性，为神经退行性疾病机制提供分子水平证据。
2. **文献名称**: *Structural analysis of pathogenic TUBB4A variants using cryo-EM*
**作者**: Smith, J.R. & Wang, L.
**摘要**: 通过冷冻电镜解析 TUBB4A 突变体重组蛋白的三维结构，揭示突变导致微管原丝间相互作用减弱，阐明了 TUBB4A 相关脑白质营养不良的潜在病理机制。
3. **文献名称**: *Recombinant TUBB4A expression rescues microtubule defects in patient-derived iPSC models*
**作者**: Johnson, M. et al.
**摘要**: 在患者诱导多能干细胞(iPSCs)中表达野生型重组 TUBB4A 蛋白，成功逆转神经元微管紊乱表型，证明基因治疗在 TUBB4A 突变相关疾病中的潜力。
4. **文献名称**: *High-throughput screening of TUBB4A-targeted small molecule stabilizers*
**作者**: Patel, S. et al.
**摘要**: 基于重组 TUBB4A 蛋白建立药物筛选平台，鉴定出可增强突变蛋白微管结合能力的小分子化合物，为开发针对 TUBB4A 疾病的靶向疗法提供候选分子。
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注：以上文献为示例，实际研究需参考真实发表的论文(如 PubMed 或 Web of Science 数据库)。
**Background of TUBB4A Recombinant Protein**
TUBB4A, a member of the β-tubulin family, is a critical component of microtubules—dynamic cytoskeletal structures essential for cell division, intracellular transport, and maintaining cell shape. This protein is particularly abundant in the central nervous system, where it supports neuronal migration, axon guidance, and synaptic function. Mutations in the *TUBB4A* gene are linked to neurological disorders, including hypomyelination with atrophy of the basal ganglia and cerebellum (H-ABC), dystonia, and leukodystrophies, highlighting its role in neural development and homeostasis.
Recombinant TUBB4A protein is engineered using biotechnological methods, often expressed in bacterial or mammalian systems to ensure high purity and functionality. Researchers utilize this protein to study microtubule dynamics, investigate disease mechanisms, and screen therapeutic compounds targeting tubulin-related pathologies. Its recombinant form enables precise control over post-translational modifications, which are crucial for mimicking native tubulin behavior in vitro.
Applications extend to structural studies, such as cryo-EM or X-ray crystallography, to resolve how TUBB4A mutations disrupt microtubule assembly or stability. Additionally, it serves as a tool for developing gene therapies or small-molecule interventions for TUBB4A-linked disorders. By providing a consistent and scalable source of the protein, recombinant TUBB4A accelerates both basic research and translational efforts aimed at understanding microtubule biology and addressing related diseases.
In summary, TUBB4A recombinant protein bridges molecular insights into tubulin dysfunction with clinical innovations, offering a versatile platform for advancing neuroscience and therapeutic development.
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