关键信息
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基因名
FARSA
- 应用
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别名
FARSA；FARS；FARSL；FARSLA；Phenylalanine--tRNA ligase alpha subunit
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9Y285
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表达区间
2-508aa
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氨基酸序列
ADGQVAELLLRRLEASDGGLDSAELAAELGMEHQAVVGAVKSLQALGEVIEAELRSTKHWELTAEGEEIAREGSHEARVFRSIPPEGLAQSELMRLPSGKVGFSKAMSNKWIRVDKSAADGPRVFRVVDSMEDEVQRRLQLVRGGQAEKLGEKERSELRKRKLLAEVTLKTYWVSKGSAFSTSISKQETELSPEMISSGSWRDRPFKPYNFLAHGVLPDSGHLHPLLKVRSQFRQIFLEMGFTEMPTDNFIESSFWNFDALFQPQQHPARDQHDTFFLRDPAEALQLPMDYVQRVKRTHSQGGYGSQGYKYNWKLDEARKNLLRTHTTSASARALYRLAQKKPFTPVKYFSIDRVFRNETLDATHLAEFHQIEGVVADHGLTLGHLMGVLREFFTKLGITQLRFKPAYNPYTEPSMEVFSYHQGLKKWVEVGNSGVFRPEMLLPMGLPENVSVIAWGLSLERPTMIKYGINNIRELVGHKVNLQMVYDSPLCRLDAEPRPPPTQEAA
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分子量
73.4 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
FARSA, or phenylalanyltRNA synthetase, is an essential enzyme involved in protein synthesis, specifically responsible for attaching the amino acid phenylalanine to its corresponding transfer RNA (tRNA). This process is crucial for translating genetic information into functional proteins. The importance of FARSA extends beyond its fundamental role in protein synthesis; it is also linked to various cellular processes and metabolic pathways. Dysregulation or mutations in FARSA can lead to severe consequences, including metabolic disorders and diseases. Recent research has focused on the structural and functional characterization of FARSA, including the development of recombinant FARSA proteins. These studies aim to elucidate the enzyme's mechanism of action, explore its interactions with tRNA and other proteins, and investigate its potential as a target for therapeutic intervention. The recombinant expression of FARSA allows for detailed biochemical assays and provides a platform for high-throughput screening of small molecule inhibitors, which could pave the way for novel treatments for related diseases. As protein engineering techniques advance, FARSA serves as a model for understanding broader aspects of aminoacyl-tRNA synthetases, contributing valuable insights into the intricate web of cellular biology and its implications for health and disease.
