Cat: IPD-X41725

Recombinant Streptococcus mutans serotype c gtfC Protein ,His & Myc

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关键信息

  • 基因名

    gtfC

  • 应用

    SPRMSTBLIITCELISA细胞实验药物筛选

  • 别名

    (GTF-SI)(Dextransucrase)(Sucrose 6-glucosyltransferase)

  • 种属

    Streptococcus mutans serotype c

  • 表达系统

    E. coli

  • 标签

    N- His & C- Myc

  • 纯度

    Greater than 90% as determined by SDS-PAGE.

  • 蛋白编号

    P13470

  • 表达区间

    426-597aa

  • 分子量

    26.7 kDa

  • 内毒素

    < 1.0 EU per μg protein as determined by the LAL method.

  • 性状

    Freeze-dried powder

  • 缓冲液

    PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

  • 复溶方法

    Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

  • 个性化定制

    点位突变 标签定制 buffer定制 全长蛋白定制

  • 稳定性测试

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.

  • 保存条件 & 期限

    Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

  • 运输条件

    In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.

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背景信息

GtfC, a crucial enzyme identified in the biosynthetic pathway of glucosyltransferases, plays a significant role in the production of exopolysaccharides in various microorganisms. These exopolysaccharides are important for their applications in food, pharmaceuticals, and biotechnology. Previous studies have indicated that GtfC is involved in the modification of polysaccharides, impacting their texture, viscosity, and functional properties. Researchers have focused on the recombinant expression of GtfC to explore its characteristics, optimize production processes, and comprehend its mechanisms of action. The recombinant protein offers a valuable model for elucidating the enzyme's structure-function relationship and for investigating its potential applications in industrial settings. With advancements in molecular biology techniques, including gene cloning and expression systems, scientists can produce GtfC in larger quantities, facilitating detailed biochemical studies and practical applications. Understanding GtfC's functionality not only sheds light on microbial polysaccharide synthesis but also opens up avenues for engineering enhanced strains for biotechnological purposes, such as improved fermentation processes or the development of novel materials with specific properties.

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