| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZBTB9 |
| Uniprot No | Q96C00 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-473aa |
| 氨基酸序列 | METPTPLPPVPASPTCNPAPRTIQIEFPQHSSSLLESLNRHRLEGKFCDV SLLVQGRELRAHKAVLAAASPYFHDKLLLGDAPRLTLPSVIEADAFEGLL QLIYSGRLRLPLDALPAHLLVASGLQMWQVVDQCSEILRELETSGGGISA RGGNSYHALLSTTSSTGGWCIRSSPFQTPVQSSASTESPASTESPVGGEG SELGEVLQIQVEEEEEEEEDDDDEDQGSATLSQTPQPQRVSGVFPRPHGP HPLPMTATPRKLPEGESAPLELPAPPALPPKIFYIKQEPFEPKEEISGSG TQPGGAKEETKVFSGGDTEGNGELGFLLPSGPGPTSGGGGPSWKPVDLHG NEILSGGGGPGGAGQAVHGPVKLGGTPPADGKRFGCLCGKRFAVKPKRDR HIMLTFSLRPFGCGICNKRFKLKHHLTEHMKTHAGALHACPHCGRRFRVH ACFLRHRDLCKGQGWATAHWTYKLEHHHHHH |
| 预测分子量 | 52 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ZBTB9重组蛋白的3篇示例参考文献(注：以下为虚拟文献，实际研究中请根据具体论文调整)：
1. **文献名称**：*"ZBTB9 regulates oncogenic signaling in colorectal cancer through recombinant protein-mediated pathway inhibition"*
**作者**：Chen L, et al.
**摘要**：本研究利用重组ZBTB9蛋白，揭示其通过抑制Wnt/β-catenin信号通路抑制结直肠癌细胞增殖的分子机制，为靶向治疗提供新策略。
2. **文献名称**：*"Structural characterization of ZBTB9 recombinant protein and its DNA-binding specificity"*
**作者**：Kim S, et al.
**摘要**：通过X射线晶体学解析ZBTB9重组蛋白结构，发现其锌指结构域特异性地识别DNA甲基化区域，调控表观遗传相关基因表达。
3. **文献名称**：*"ZBTB9 recombinant protein modulates macrophage polarization in inflammatory diseases"*
**作者**：Wang Y, et al.
**摘要**：体外实验表明，重组ZBTB9蛋白通过调控NF-κB活性，促进巨噬细胞向抗炎表型转化，缓解小鼠模型中的炎症反应。
(提示：若需真实文献，建议在PubMed或Web of Science中检索关键词"ZBTB9 recombinant protein"或结合具体研究领域筛选。)
ZBTB9. a member of the zinc finger and BTB domain-containing (ZBTB) protein family, is a transcription factor implicated in regulating gene expression and cellular processes. The ZBTB family proteins are characterized by two conserved domains: an N-terminal BTB/POZ domain involved in protein-protein interactions and dimerization, and C-terminal zinc finger motifs that mediate DNA binding. ZBTB9 specifically contains multiple C2H2-type zinc fingers, suggesting its role in sequence-specific DNA recognition and transcriptional modulation. Although its biological functions remain less characterized compared to other ZBTB members, emerging studies link ZBTB9 to developmental pathways, cell differentiation, and potential involvement in diseases such as cancer or neurological disorders.
Recombinant ZBTB9 protein is typically produced using heterologous expression systems (e.g., E. coli or mammalian cells) to enable functional and structural studies. The recombinant form often includes affinity tags (e.g., His-tag, GST) for purification and detection. Researchers utilize this protein to investigate its DNA-binding specificity, interaction partners (e.g., chromatin remodelers or co-repressors), and regulatory mechanisms. In vitro assays, such as electrophoretic mobility shift assays (EMSAs) or chromatin immunoprecipitation (ChIP)-seq, have been employed to map target genes and elucidate its role in transcriptional networks.
Current research focuses on clarifying ZBTB9's physiological roles, particularly its interplay with signaling pathways like Wnt/β-catenin or Notch, which are critical in development and disease. Its recombinant form also serves as an antigen for antibody development or a control in diagnostic applications. Further exploration of ZBTB9 may uncover novel therapeutic targets, given the ZBTB family's established connections to immune regulation and oncogenesis. However, limited in vivo data necessitate deeper mechanistic studies to validate its functional relevance across biological contexts.
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