关键信息
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基因名
ORC1
- 应用
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别名
(Replication control protein 1)
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种属
Human
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表达系统
E. coli
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标签
N- GST
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q13415
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表达区间
1-861aa
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分子量
123.6 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
ORC1, or Origin Recognition Complex Subunit 1, plays a crucial role in DNA replication and cell cycle regulation by initiating the formation of the pre-replicative complex at replication origins. This protein is part of the larger ORC complex, which is essential for the licensing of DNA replication, ensuring that DNA is replicated once and only once per cell cycle. Research has shown that ORC1 is not only pivotal for maintaining genomic stability but also has implications in various cellular processes, including transcriptional regulation and chromatin organization. Dysregulation of ORC1 has been associated with several diseases, including cancer, where aberrant replication can lead to genomic instability and tumorigenesis. Given its vital functions, ORC1 thus serves as a potential target for therapeutic intervention. Studies involving recombinant ORC1 protein have been instrumental in elucidating its structure-function relationship, interactions with other replication factors, and the mechanisms underlying its regulation during the cell cycle. Through advances in protein engineering and purification techniques, researchers have been able to generate substantial amounts of recombinant ORC1, facilitating in-depth biochemical studies and structural analyses. This research is critical for understanding the fundamental processes of DNA replication and the potential roles of ORC1 in pathological conditions, paving the way for novel strategies in cancer treatment and the broader field of cell biology.
