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Recombinant Human MDH2 protein

  • 中文名: 苹果酸脱氢酶2(MDH2)重组蛋白
  • 别    名: MDH2;Malate dehydrogenase, mitochondrial
货号: PA1000-5094
Price: ¥询价
数量:
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产品详情

纯度>90%SDS-PAGE.
种属Human
靶点MDH2
Uniprot No P40926
内毒素< 0.01EU/μg
表达宿主E.coli
表达区间25-338aa
氨基酸序列AKVAVLGASGGIGQPLSLLLKNSPLVSRLTLYDIAHTPGVAADLSHIETKAAVKGYLGPEQLPDCLKGCDVVVIPAGVPRKPGMTRDDLFNTNATIVATLTAACAQHCPEAMICVIANPVNSTIPITAEVFKKHGVYNPNKIFGVTTLDIVRANTFVAELKGLDPARVNVPVIGGHAGKTIIPLISQCTPKVDFPQDQLTALTGRIQEAGTEVVKAKAGAGSATLSMAYAGARFVFSLVDAMNGKEGVVECSFVKSQETECTYFSTPLLLGKKGIEKNLGIGKVSSFEEKMISDAIPELKASIKKGEDFVKTLK
预测分子量 49.0kDa
蛋白标签His tag N-Terminus
缓冲液PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.
Reconstituted protein solution can be stored at 2-8°C for 2-7 days.
Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于MDH2重组蛋白的参考文献示例(注:以下为模拟文献,仅供参考):

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1. **文献名称**:*Recombinant Expression and Crystallographic Analysis of Human Mitochondrial MDH2*

**作者**:Zhang Y, et al.

**摘要**:报道了人源MDH2重组蛋白在大肠杆菌中的高效表达与纯化,并通过X射线晶体学解析其三维结构,揭示了底物结合位点的关键氨基酸残基。

2. **文献名称**:*Functional Characterization of MDH2 Mutants Using Recombinant Protein Assays*

**作者**:Kim H, et al.

**摘要**:通过重组MDH2蛋白的体外实验,分析了癌症相关突变体(如R140Q)的酶活性和热稳定性变化,探讨其对三羧酸循环的潜在影响。

3. **文献名称**:*Development of a High-Throughput Screening Platform for MDH2 Inhibitors Based on Recombinant Protein*

**作者**:Garcia M, et al.

**摘要**:建立基于重组MDH2蛋白的抑制剂筛选体系,鉴定出多个可抑制线粒体代谢通路的小分子化合物,为抗癌药物开发提供候选分子。

4. **文献名称**:*MDH2 Recombinant Protein as a Diagnostic Marker for Mitochondrial Disorders*

**作者**:Patel S, et al.

**摘要**:利用重组MDH2蛋白开发抗体检测工具,用于临床样本中MDH2表达水平的定量分析,辅助诊断线粒体功能障碍相关疾病。

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**备注**:以上文献为示例性内容,实际引用时建议通过PubMed、Web of Science等数据库检索关键词“MDH2 recombinant protein”“malate dehydrogenase 2 expression”获取真实文献。

背景信息

Malate dehydrogenase 2 (MDH2) is a mitochondrial enzyme critical to the tricarboxylic acid (TCA) cycle, catalyzing the reversible oxidation of malate to oxaloacetate while reducing NAD⁺ to NADH. This reaction links carbohydrate, lipid, and amino acid metabolism, playing a pivotal role in cellular energy production and redox balance. MDH2 dysfunction has been implicated in metabolic disorders, neurodegenerative diseases, and cancer, making it a target for therapeutic research.

Recombinant MDH2 protein is engineered using genetic cloning techniques to enable large-scale production for biochemical and medical studies. The gene encoding human MDH2 is inserted into expression vectors (e.g., bacterial, yeast, or mammalian systems) to produce tagged or untagged versions. Escherichia coli-based systems are commonly employed due to their cost-effectiveness and high yield, though post-translational modifications may require eukaryotic hosts. Purification typically involves affinity chromatography (e.g., His-tag), followed by enzymatic activity validation.

Research applications include elucidating MDH2’s structural dynamics, substrate specificity, and regulatory mechanisms. Its role in metabolic reprogramming in cancer cells—where altered TCA flux supports tumor growth—has driven interest in developing MDH2 inhibitors. Additionally, recombinant MDH2 serves as a tool for diagnosing mitochondrial disorders or screening small molecules targeting metabolic syndromes. Recent studies also explore its interaction with other TCA enzymes and apoptotic proteins, highlighting broader implications in cellular homeostasis. The availability of recombinant MDH2 accelerates functional studies and drug discovery efforts aimed at metabolic and age-related diseases.

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