关键信息
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基因名
EIF3I
- 应用
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别名
EIF3I；EIF3S2；TRIP1;Eukaryotic translation initiation factor 3 subunit I
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q13347
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表达区间
1-325aa
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氨基酸序列
MKPILLQGHERSITQIKYNREGDLLFTVAKDPIVNVWYSVNGERLGTYMGHTGAVWCVDADWDTKHVLTGSADNSCRLWDCETGKQLALLKTNSAVRTCGFDFGGNIIMFSTDKQMGYQCFVSFFDLRDPSQIDNNEPYMKIPCNDSKITSAVWGPLGECIIAGHESGELNQYSAKSGEVLVNVKEHSRQINDIQLSRDMTMFVTASKDNTAKLFDSTTLEHQKTFRTERPVNSAALSPNYDHVVLGGGQEAMDVTTTSTRIGKFEARFFHLAFEEEFGRVKGHFGPINSVAFHPDGKSYSSGGEDGYVRIHYFDPQYFEFEFEA
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分子量
39 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
EIF3I, or Eukaryotic Translation Initiation Factor 3 Subunit I, plays a critical role in the initiation of protein synthesis, functioning as a component of the eukaryotic translation initiation factor 3 (eIF3) complex. This complex is essential for bridging the small ribosomal subunit and the mRNA, facilitating the assembly of the translation machinery at the start codon. Research has shown that EIF3I is not only vital for normal cell growth and development but also implicated in various pathophysiological conditions, including cancer. Abnormal expression levels of EIF3I have been associated with tumor progression, suggesting that it may influence the translation of specific mRNAs involved in cell proliferation and survival. As a result, there is significant interest in studying EIF3I and its interactions within the translational machinery to better understand its role in cancer biology and to explore its potential as a therapeutic target. Advances in recombinant protein expression techniques have enabled the production of EIF3I for structural and functional analyses, facilitating the determination of its precise role in translation initiation and its interactions with other eIF3 subunits and factors. This research could pave the way for novel strategies in cancer treatment and provide insights into the regulation of gene expression at the translational level.
