关键信息
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基因名
EIF4H
- 应用
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别名
EIF4H；KIAA0038；WBSCR1；WSCR1;Eukaryotic translation initiation factor 4H
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q15056
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表达区间
1-248aa
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氨基酸序列
MGSSHHHHHH SSGLVPRGSH MGSHMADFDT YDDRAYSSFG GGRGSRGSAG GHGSRSQKEL PTEPPYTAYV GNLPFNTVQG DIDAIFKDLS IRSVRLVRDK DTDKFKGFCY VEFDEVDSLK EALTYDGALL GDRSLRVDIA EGRKQDKGGF GFRKGGPDDR GMGSSRESRG GWDSRDDFNS GFRDDFLGGR GGSRPGDRRT GPPMGSRFRD GPPLRGSNMD FREPTEEERA QRPRLQLKPR TVATPLNQVA NPNSAIFGGA RPREEVVQKE QE
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分子量
30 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
EIF4H, or Eukaryotic Initiation Factor 4H, is a crucial component in the protein synthesis machinery of eukaryotic cells. It is involved in the initiation phase of translation by interacting with the RNA cap structure and unwinding secondary RNA structures, thereby facilitating the binding of ribosomes to mRNA. Since its involvement in translation regulation is vital for cellular growth and response to environmental stimuli, studying EIF4H has significant implications for understanding various biological processes, including cancer progression and viral infections, where hijacking the host translational machinery is common. Additionally, mutations or dysregulation of EIF4H have been associated with several diseases, highlighting its potential as a therapeutic target. The production of EIF4H recombinant proteins using techniques such as recombinant DNA technology allows for detailed structural and functional studies, providing insights into its role in translation and potential interactions with other cellular factors. This research is essential not only for basic biological understanding but also for developing novel strategies in drug design and therapeutic interventions. As such, the study of EIF4H recombinant proteins is an emerging field that holds promise for uncovering new aspects of gene regulation and cellular metabolism.
