关键信息
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基因名
egfp
- 应用
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别名
egfp；CREME9；CRLM9；CYTOR4；Cytokine receptor-like factor 3
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
C5MKY7
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表达区间
1-239aa
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氨基酸序列
MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK
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分子量
28.4 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
The enhanced Green Fluorescent Protein (eGFP) has become a pivotal tool in molecular and cellular biology since its introduction as an improved variant of the original Green Fluorescent Protein (GFP) derived from the jellyfish Aequorea victoria. eGFP exhibits increased brightness and stability, making it a preferred marker for visualizing and tracking cellular processes in live cells. Researchers utilize eGFP in a wide range of applications, including gene expression studies, protein localization, and dynamic imaging of cellular interactions. Its non-toxic nature allows for the observation of biological processes in real time without disrupting cellular function. The versatility of eGFP has led to its incorporation into various experimental techniques such as fluorescence microscopy, flow cytometry, and live-cell imaging. Moreover, advancements in eGFP technology have enabled the development of fusion proteins, where eGFP is linked to other proteins of interest, facilitating the study of protein interactions and dynamics within the cellular environment. As research continues to expand, the application of eGFP in genetic engineering, transgenic organisms, and drug discovery remains significant, highlighting its role as a vital tool in advancing our understanding of complex biological systems.
