关键信息
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基因名
TK1
- 应用
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别名
TK1；Thymidine kinase. cytosolic
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P04183
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表达区间
1-234aa
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氨基酸序列
MGSSHHHHHH SSGLVPRGSH MGSHMSCINL PTVLPGSPSK TRGQIQVILG PMFSGKSTEL MRRVRRFQIA QYKCLVIKYA KDTRYSSSFC THDRNTMEAL PACLLRDVAQ EALGVAVIGI DEGQFFPDIV EFCEAMANAG KTVIVAALDG TFQRKPFGAI LNLVPLAESV VKLTAVCMEC FREAAYTKRL GTEKEVEVIG GADKYHSVCR LCYFKKASGQ PAGPDNKENC PVPGKPGEAV AARKLFAPQQ ILQCSPAN
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分子量
28 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
The study of TK1 (thymidine kinase 1) recombinant protein is driven by its significant role in cellular proliferation and its potential applications in cancer therapy and diagnostics. TK1 is an enzyme involved in the salvage pathway of nucleotide synthesis, specifically converting thymidine into thymidine monophosphate, which is crucial for DNA synthesis and repair. Elevated levels of TK1 are often associated with increased cell turnover and are observed in various malignancies, making it a valuable biomarker for cancer progression and prognosis. The recombinant expression of TK1 allows for the production of large quantities of the protein, facilitating detailed studies of its biological functions and interactions. Additionally, understanding the structure and dynamics of TK1 can aid in the design of inhibitors that may serve as therapeutic agents targeting cancer cell metabolism. The development of TK1 as a diagnostic marker has led to innovations in biomarker assays, improving early detection and monitoring of cancer therapy. Overall, research into TK1 recombinant protein not only enhances our understanding of cancer biology but also paves the way for novel diagnostic and therapeutic strategies in oncology.
