关键信息
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基因名
DYNC1I1
- 应用
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别名
DYNC1I1；DNCI1；DNCIC1；Cytoplasmic dynein 1 intermediate chain 1
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
O14576
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表达区间
1-645aa
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氨基酸序列
MSDKSDLKAELERKKQRLAQIREEKKRKEEERKKKEADMQQKKEPVQDDSDLDRKRRETEALLQSIGISPEPPLVQPLHFLTWDTCYFHYLVPTPMSPSSKSVSTPSEAGSQDSGDLGPLTRTLQWDTDPSVLQLQSDSELGRRLHKLGVSKVTQVDFLPREVVSYSKETQTPLATHQSEEDEEDEEMVESKVGQDSELENQDKKQEVKEAPPRELTEEEKQQILHSEEFLIFFDRTIRVIERALAEDSDIFFDYSGRELEEKDGDVQAGANLSFNRQFYDEHWSKHRVVTCMDWSLQYPELMVASYNNNEDAPHEPDGVALVWNMKFKKTTPEYVFHCQSSVMSVCFARFHPNLVVGGTYSGQIVLWDNRSHRRTPVQRTPLSAAAHTHPVYCVNVVGTQNAHNLITVSTDGKMCSWSLDMLSTPQESMELVYNKSKPVAVTGMAFPTGDVNNFVVGSEEGTVYTACRHGSKAGIGEVFEGHQGPVTGINCHMAVGPIDFSHLFVTSSFDWTVKLWTTKHNKPLYSFEDNADYVYDVMWSPVHPALFACVDGMGRLDLWNLNNDTEVPTASVAIEGASALNRVRWAQAGKEVAVGDSEGRIWVYDVGELAVPHNDEWTRFARTLVEIRANRADSEEEGTVELSA
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分子量
75.4 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
DYNC1I1, or cytoplasmic dynein 1 heavy chain 1, is a critical component of the dynein motor protein complex, which is essential for many cellular processes, including organelle transport, mitosis, and endocytosis. Research into DYNC1I1 has gained momentum due to its significant role in cellular machinery and its association with various neurodegenerative diseases, such as Alzheimer's and Huntington's disease, where disrupted intracellular transport leads to cellular dysfunction. Genetic mutations or dysregulation in DYNC1I1 expression can impair dynein function, resulting in compromised neuronal health and tissue homeostasis. As a result, the study of DYNC1I1 recombinant proteins offers valuable insights into the mechanistic pathways of motor protein function and its implications in disease models. By expressing and characterizing DYNC1I1 in a recombinant system, researchers can investigate the structural and functional properties of this protein, evaluate its interactions with other cellular components, and screen potential therapeutic compounds that could restore normal dynein activity. Ultimately, understanding DYNC1I1’s biology is pivotal for developing targeted interventions in diseases linked to dynein malfunction, making it a focal point of cellular and biomedical research.
