关键信息
-
基因名
GPX4（U73C）
- 应用
-
别名
PHGPx;Glutathione peroxidase 4;GPx-4;GSHPx-4
-
种属
Human
-
表达系统
E. coli
-
标签
His tag N-Terminus
-
纯度
Greater than 90% as determined by SDS-PAGE.
-
蛋白编号
P36969
-
表达区间
29-197aa
-
氨基酸序列
CASRDDWRCARSMHEFSAKDIDGHMVNLDKYRGFVCIVTNVASQSGKTEVNYTQLVDLHARYAECGLRILAFPCNQFGKQEPGSNEEIKEFAAGYNVKFDMFSKICVNGDDAHPLWKWMKIQPKGKGILGNAIKWNFTKFLIDKNGCVVKRYGPMEEPLVIEKDLPHYF
-
分子量
21.4 kDa
-
内毒素
< 1.0 EU per μg protein as determined by the LAL method.
-
性状
Freeze-dried powder
-
缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
-
稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
GPX4 (Glutathione Peroxidase 4) is a critical enzyme that plays a vital role in regulating redox homeostasis and lipid metabolism within cells. Unlike other members of the GPX family, GPX4 is unique in its ability to reduce hydroperoxides and protect cells from oxidative damage, particularly in maintaining the integrity of membrane lipids. Its activity is essential for cellular survival, particularly in preventing ferroptosis, a form of regulated cell death driven by lipid peroxidation. Research into the recombinant expression of GPX4 (specifically the U73C variant) has gained traction due to its potential therapeutic implications in various diseases, including cancer and neurodegenerative disorders. The recombinant protein serves as a valuable tool for understanding the molecular mechanisms underpinning its antioxidant functions and for evaluating its therapeutic efficacy in mitigating oxidative stress-related damage. By producing GPX4 in a recombinant system, researchers are able to obtain large quantities of the protein for biochemical studies and potential drug development, aiming to harness its protective effects against oxidative damage, with the ultimate goal of designing novel interventions for diseases characterized by oxidative stress and ferroptosis.
