Cat: PA2000-3944

Recombinant E.coli GRA3 Protein,His

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关键信息

  • 基因名

    GRA3

  • 应用

    SPRMSTBLIITCELISA细胞实验药物筛选

  • 别名

    GRA3;Glycine receptor subunit alpha-3

  • 种属

    E.coli

  • 表达系统

    E. coli

  • 标签

    His tag N-Terminus

  • 纯度

    Greater than 90% as determined by SDS-PAGE.

  • 蛋白编号

    B6KEU8

  • 表达区间

    43-114aa

  • 氨基酸序列

    ADQPENHQALAEPVTGVGEAGVSPVNEAGESYSSATSGVQEATAPGAVLLDAIDAESDKVDNQAEGGERMKK

  • 分子量

    10.8 kDa

  • 内毒素

    < 1.0 EU per μg protein as determined by the LAL method.

  • 性状

    Freeze-dried powder

  • 缓冲液

    PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

  • 复溶方法

    Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

  • 个性化定制

    点位突变 标签定制 buffer定制 全长蛋白定制

  • 稳定性测试

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.

  • 保存条件 & 期限

    Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

  • 运输条件

    In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.

质检流程

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背景信息

GRA3, or GRA3 protein, is an important component of the parasitic organism Toxoplasma gondii, which is known for its significant impact on human health and agriculture. T. gondii is an intracellular parasite that can cause toxoplasmosis, a disease that affects millions of people worldwide, particularly immunocompromised individuals and pregnant women. GRA3 is part of a group of proteins known as “Dense Granule Antigens” (GRA), which are secreted by the parasite during its invasion of host cells. These proteins play a crucial role in modulating the host's immune response and establishing an intracellular niche conducive to the parasite's survival. Research on GRA3 has gained attention due to its potential as a target for vaccine development and therapeutic intervention, as understanding its structure and function may reveal insights into the parasite's biology and its interactions with the host immune system. Studies have shown that GRA3 can influence various signaling pathways within the host cells, thus helping the parasite evade immune detection and enhancing its pathogenicity. Moreover, the investigation of GRA3's molecular characteristics may provide valuable information for the development of diagnostic tools and novel treatment strategies, particularly in regions with high prevalence rates of T. gondii infections. As such, ongoing research into GRA3 is critical for addressing the public health challenges posed by toxoplasmosis and advancing our understanding of host-parasite interactions in the context of infectious diseases.

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