| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TCN2 |
| Uniprot No | P20062-1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-427aa |
| 氨基酸序列 | MRHLGAFLFL LGVLGALTEM CEIPEMDSHL VEKLGQHLLP WMDRLSLEHL NPSIYVGLRL SSLQAGTKED LYLHSLKLGY QQCLLGSAFS EDDGDCQGKP SMGQLALYLL ALRANCEFVR GHKGDRLVSQ LKWFLEDEKR AIGHDHKGHP HTSYYQYGLG ILALCLHQKR VHDSVVDKLL YAVEPFHQGH HSVDTAAMAG LAFTCLKRSN FNPGRRQRIT MAIRTVREEI LKAQTPEGHF GNVYSTPLAL QFLMTSPMRG AELGTACLKA RVALLASLQD GAFQNALMIS QLLPVLNHKT YIDLIFPDCL APRVMLEPAA ETIPQTQEII SVTLQVLSLL PPYRQSISVL AGSTVEDVLK KAHELGGFTY ETQASLSGPY LTSVMGKAAG EREFWQLLRD PNTPLLQGIA DYRPKDGETI ELRLVSW |
| 预测分子量 | 47 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于TCN2(转钴胺素II)重组蛋白研究的模拟参考文献示例(文献信息为虚构，仅供格式参考，实际文献需通过学术数据库查询)：
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1. **文献名称**：*Expression and Purification of Recombinant Human TCN2 in Escherichia coli for Vitamin B12 Binding Studies*
**作者**：Smith J, et al.
**摘要**：研究报道了在大肠杆菌中高效表达可溶性重组人TCN2蛋白的优化方法，并通过亲和层析纯化获得高纯度蛋白。实验证实重组TCN2能够特异性结合维生素B12.为其在代谢疾病研究中的应用提供基础。
2. **文献名称**：*Functional Characterization of TCN2 Mutants Expressed in HEK293 Cells: Implications for Genetic Deficiency Disorders*
**作者**：Lee H, et al.
**摘要**：通过在HEK293哺乳动物细胞中表达野生型及突变型TCN2重组蛋白，分析了不同突变对维生素B12转运效率的影响，揭示了某些遗传性TCN2缺陷导致维生素B12代谢异常的分子机制。
3. **文献名称**：*Development of a TCN2-Based Fluorescent Probe for Cellular Vitamin B12 Uptake Imaging*
**作者**：Wang Y, et al.
**摘要**：利用重组TCN2蛋白与荧光标记技术开发了一种新型探针，成功实现活细胞内维生素B12动态摄取的实时追踪，为相关疾病诊断提供了工具。
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**提示**：实际研究中，建议通过PubMed、Google Scholar等平台以关键词“recombinant TCN2 protein”、“transcobalamin II expression”检索最新文献，并优先选择近五年内发表的实验性研究论文。
TCN2 (Transcobalamin II) is a essential plasma glycoprotein responsible for the transport of vitamin B12 (cobalamin), a critical cofactor in DNA synthesis, hematopoiesis, and neurological function. Encoded by the TCN2 gene, this 45 kDa protein binds and delivers dietary cobalamin to cells via receptor-mediated endocytosis, ensuring cellular uptake through interactions with the CD320 receptor. Unlike other cobalamin-binding proteins, TCN2 represents the biologically active fraction of circulating vitamin B12. making it a key biomarker for assessing functional vitamin B12 status.
Recombinant TCN2 protein is produced using engineered expression systems (e.g., mammalian cells or E. coli) to overcome limitations of native protein purification from blood plasma. This biotechnology approach enables scalable production with consistent post-translational modifications, particularly important for maintaining ligand-binding capacity. The recombinant form retains the ability to form complexes with cobalamin and interact with cellular receptors, mirroring endogenous functionality.
Research applications span multiple domains: as a tool to study vitamin B12 metabolism disorders, a potential therapeutic for cobalamin delivery in genetic deficiencies, and a diagnostic reagent for autoimmune conditions involving anti-TCN2 antibodies. Its role in cellular proliferation has also spurred interest in cancer research, particularly in malignancies with elevated TCN2 expression. Furthermore, recombinant TCN2 facilitates standardized assays for vitamin B12 deficiency diagnosis, addressing variability in traditional clinical measurements. Ongoing studies explore its utility in drug delivery systems and as a biomarker for neurodegenerative diseases linked to impaired cobalamin transport.
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